pBABEpuro HA Brca1 | Addgene 14999 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBABEpuro HA Brca1

catalog :

14999

citations: 11

Reference
Wang Q, Bergholz J, Ding L, Lin Z, Kabraji S, Hughes M, et al. STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer. Nat Commun. 2022;13:3022 pubmed publisher
Park J, Kang J, Hahm J, Kim H, Seo S. Proteosomal degradation of NSD2 by BRCA1 promotes leukemia cell differentiation. Commun Biol. 2020;3:462 pubmed publisher
Kurihara M, Mano T, Saito Y, Murayama S, Toda T, Iwata A. Colocalization of BRCA1 with Tau Aggregates in Human Tauopathies. Brain Sci. 2019;10: pubmed publisher
Li Q, Hao Q, Cao W, Li J, Wu K, Elshimali Y, et al. PP2Cδ inhibits p300-mediated p53 acetylation via ATM/BRCA1 pathway to impede DNA damage response in breast cancer. Sci Adv. 2019;5:eaaw8417 pubmed publisher
Biswas K, Philip S, Yadav A, Martin B, Burkett S, Singh V, et al. BRE/BRCC45 regulates CDC25A stability by recruiting USP7 in response to DNA damage. Nat Commun. 2018;9:537 pubmed publisher
Chiyoda T, Hart P, Eckert M, McGregor S, Lastra R, Hamamoto R, et al. Loss of BRCA1 in the Cells of Origin of Ovarian Cancer Induces Glycolysis: A Window of Opportunity for Ovarian Cancer Chemoprevention. Cancer Prev Res (Phila). 2017;10:255-266 pubmed publisher
Luo J, Jin J, Yang F, Sun Z, Zhang W, Shi Y, et al. The Correlation Between PARP1 and BRCA1 in AR Positive Triple-negative Breast Cancer. Int J Biol Sci. 2016;12:1500-1510 pubmed
Zhang W, Luo J, Yang F, Wang Y, Yin Y, Strom A, et al. BRCA1 inhibits AR-mediated proliferation of breast cancer cells through the activation of SIRT1. Sci Rep. 2016;6:22034 pubmed publisher
Fan S, Liu B, Sun L, Lv X, Lin Z, Chen W, et al. Mitochondrial fission determines cisplatin sensitivity in tongue squamous cell carcinoma through the BRCA1-miR-593-5p-MFF axis. Oncotarget. 2015;6:14885-904 pubmed
Zhang W, Luo J, Chen F, Yang F, Song W, Zhu A, et al. BRCA1 regulates PIG3-mediated apoptosis in a p53-dependent manner. Oncotarget. 2015;6:7608-18 pubmed
Cortez D, Wang Y, Qin J, Elledge S. Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Science. 1999;286:1162-6 pubmed

product information

Catalog Number :

14999

Product Name :

pBABEpuro HA Brca1

article :

doi	10.1126/science.286.5442.1162
id	1421
pubmed_id	10550055

bacterial resistance :

Ampicillin

cloning :

backbone

pBABE-puro

backbone_mutation

backbone_origin

backbone_size

5200

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

pBABEpuro HABrca1 was made by digesting pCDNA3beta HABrca1 (R. Scully, et al., Cell 88, 265, 1997) with SalI and XhoI and inserting it into the SalI site of pBABEpuro. The BRCA1 insert in this plasmid contains the following common polymorphisms when compared to GenBank reference sequence NP_009225.1: P871L, E1038G, K1182R, and S1613G. These SNPs were likely present in the original cDNA isolate.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	SalI
promoter
sequencing_primer_3	pBABE 3'
sequencing_primer_5	pBABE 5'
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53
gene	BRCA1
id	672

genbank_ids

mutation

name

Brca1

shRNA_sequence

size

species

9606

Homo sapiens