pBV-Luc/Del-7 | Addgene 14968 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBV-Luc/Del-7

catalog :

14968

citations: 1

Reference
Chen C, Kang Y, Massague J. Defective repression of c-myc in breast cancer cells: A loss at the core of the transforming growth factor beta growth arrest program. Proc Natl Acad Sci U S A. 2001;98:992-9 pubmed

product information

Catalog Number :

14968

Product Name :

pBV-Luc/Del-7

article :

doi	10.1073/pnas.98.3.992
id	1228
pubmed_id	11158583

bacterial resistance :

Ampicillin

cloning :

backbone

pBV-luciferase

backbone_mutation

backbone_origin

backbone_size

4900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

-26/+334 (relative to the P2 transcription initiation site) of the c-myc promoter was amplified by PCR and subcloned into the KpnI/EcoRI site of pBV-Luc, a low basal activity luciferase reporter plasmid.

origin :

pi :

alt_names

c-myc promoter

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	KpnI
promoter
sequencing_primer_3	LucNrev
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

mutation

-26/+334 (relative to the P2 transcription initiation site) of the c-myc promoter.

name

c-myc promoter (-26/+334)

shRNA_sequence

size

360

species

9606

Homo sapiens