CS2 Flag-Smad3 (EPSM) | Addgene 14963 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

CS2 Flag-Smad3 (EPSM)

catalog :

14963

citations: 3

Reference
Zhou J, Dabiri Y, Gama Brambila R, Ghafoory S, Altinbay M, Mehrabi A, et al. pVHL-mediated SMAD3 degradation suppresses TGF-β signaling. J Cell Biol. 2022;221: pubmed publisher
Vukotic M, Nolte H, König T, Saita S, Ananjew M, Kruger M, et al. Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria. Mol Cell. 2017;67:471-483.e7 pubmed publisher
Kretzschmar M, Doody J, Timokhina I, Massague J. A mechanism of repression of TGFbeta/ Smad signaling by oncogenic Ras. Genes Dev. 1999;13:804-16 pubmed

product information

Catalog Number :

14963

Product Name :

CS2 Flag-Smad3 (EPSM)

article :

doi	10.1101/gad.13.7.804
id	1400
pubmed_id	10197981

bacterial resistance :

Ampicillin

cloning :

backbone

pCS2

backbone_mutation

backbone_origin

backbone_size

4100

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

xenopus/mammalian/avian/zebrafish

growth notes :

3SP and 1TP sites in the linker are mutated to alanine or valine (T-DV). See author's map for more information. Please note that the mutations correspond to residues 179, 204, 208, and 213 of the native Smad3 protein.

origin :

pi :

alt_names

Smad3

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	EBV rev
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3
gene	SMAD3
id	4088

genbank_ids

mutation

T178V, S203A, S207A, S212A

name

Smad3 (EPSM)

shRNA_sequence

size

1300

species

9606

Homo sapiens