This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA Flag-Smad4M
catalog :
14959
citations: 1
Reference
Kang Y, He W, Tulley S, Gupta G, Serganova I, Chen C, et al. Breast cancer bone metastasis mediated by the Smad tumor suppressor pathway. Proc Natl Acad Sci U S A. 2005;102:13909-14 pubmed
product information
Catalog Number :
14959
Product Name :
pcDNA Flag-Smad4M
article :
doi10.1073/pnas.0506517102
id1411
pubmed_id16172383
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Site directed mutagenesis in the coding sequence of Tyr162 (from TAT to TAC) and Val163 (from GTG to GTT). The mutated sequence encodes the same amino acids. See author's map for more information.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI / SalI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5CMV Forward
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesDPC4, JIP, MADH4, MYHRS
geneSMAD4
id4089
genbank_ids
mutation
nameSmad4
shRNA_sequence
size1700
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
resistance markers :
313
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA