pCMV Fcgamma RIIa IRES neo | Addgene 14948 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV Fcgamma RIIa IRES neo

catalog :

14948

citations: 4

Reference
Herron J, Hu S, Watanabe T, Nogueira A, Liu B, Kern M, et al. Actin nano-architecture of phagocytic podosomes. Nat Commun. 2022;13:4363 pubmed publisher
Gao Y, Anthony S, Yi Y, Li W, Yu Y, Yu Y. Single-Janus Rod Tracking Reveals the "Rock-and-Roll" of Endosomes in Living Cells. Langmuir. 2018;34:1151-1158 pubmed publisher
Masters T, Sheetz M, Gauthier N. F-actin waves, actin cortex disassembly and focal exocytosis driven by actin-phosphoinositide positive feedback. Cytoskeleton (Hoboken). 2016;73:180-96 pubmed publisher
Trivedi V, Zhang S, Castoreno A, Stockinger W, Shieh E, Vyas J, et al. Immunoglobulin G signaling activates lysosome/phagosome docking. Proc Natl Acad Sci U S A. 2006;103:18226-31 pubmed

product information

Catalog Number :

14948

Product Name :

pCMV Fcgamma RIIa IRES neo

article :

doi	10.1073/pnas.0609182103
id	1408
pubmed_id	17110435

bacterial resistance :

Ampicillin

cloning :

backbone

pIRESneo2

backbone_mutation

backbone_origin

Clontech

backbone_size

5300

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The FcgRIIa cDNA was amplified by RT-PCR using a Titan One Tube RT-PCR System kit (Roche, Mannheim, Germany) and a pair of primers (5'-TTGAATTCACCATGGAGACCCAAATGTCTC-3' and 5'-GCGGCCGCTTAGTTATTACTGTTGACATGG-3'). The PCR product was cloned into pCR2.1-TOPO (Invitrogen, Carlsbad, CA) to generate pAC01 and subcloned as an EcoRI NotI fragment into pIRESneo2 (Clontech, Mountain View, CA) to generate pCMV-FcgRIIa-IRES-Neo.

origin :

pi :

alt_names

FcgRIIa

Fc gamma RIIa

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	EcoRI
promoter
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, FcGR, FcgammaRIIa, IGFR2
gene	FCGR2A
id	2212

genbank_ids

mutation

name

Fcgamma RIIa

shRNA_sequence

size

1000

species

9606

Homo sapiens