This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLDLR-Luc (aka: pES7)
catalog :
14940
citations: 10
Reference
Centonze G, Natalini D, Grasso S, Morellato A, Salemme V, Piccolantonio A, et al. p140Cap modulates the mevalonate pathway decreasing cell migration and enhancing drug sensitivity in breast cancer cells. Cell Death Dis. 2023;14:849 pubmed publisher
Firpo M, LoMascolo N, Petit M, Shah P, Mounce B. Polyamines and eIF5A hypusination facilitate SREBP2 synthesis and cholesterol production leading to enhanced enterovirus attachment and infection. PLoS Pathog. 2023;19:e1011317 pubmed publisher
Fukuda M, Ogasawara Y, Hayashi H, Inoue K, Sakashita H. Resveratrol Inhibits Proliferation and Induces Autophagy by Blocking SREBP1 Expression in Oral Cancer Cells. Molecules. 2022;27: pubmed publisher
Mai S, Zhu X, Wan E, Wu S, Yonathan J, Wang J, et al. Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2. Development. 2022;149: pubmed publisher
Goedeke L, Canfrán Duque A, Rotllan N, Chaube B, Thompson B, Lee R, et al. MMAB promotes negative feedback control of cholesterol homeostasis. Nat Commun. 2021;12:6448 pubmed publisher
Choi Y, Lee S, Kim H, Lee H, Kang S, Kim T, et al. Platycodin D enhances LDLR expression and LDL uptake via down-regulation of IDOL mRNA in hepatic cells. Sci Rep. 2020;10:19834 pubmed publisher
Pham D, Bruelle C, Thi Do H, Pajanoja C, Jin C, Srinivasan V, et al. Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells. Cell Death Dis. 2019;10:537 pubmed publisher
Pham D, Do H, Bruelle C, Kukkonen J, Eriksson O, Mogollón I, et al. p75 Neurotrophin Receptor Signaling Activates Sterol Regulatory Element-binding Protein-2 in Hepatocyte Cells via p38 Mitogen-activated Protein Kinase and Caspase-3. J Biol Chem. 2016;291:10747-58 pubmed publisher
Do H, Bruelle C, Pham D, Jauhiainen M, Eriksson O, Korhonen L, et al. Nerve growth factor (NGF) and pro-NGF increase low-density lipoprotein (LDL) receptors in neuronal cells partly by different mechanisms: role of LDL in neurite outgrowth. J Neurochem. 2016;136:306-15 pubmed publisher
Castoreno A, Wang Y, Stockinger W, Jarzylo L, Du H, Pagnon J, et al. Transcriptional regulation of phagocytosis-induced membrane biogenesis by sterol regulatory element binding proteins. Proc Natl Acad Sci U S A. 2005;102:13129-34 pubmed
product information
Catalog Number :
14940
Product Name :
pLDLR-Luc (aka: pES7)
article :
doi10.1073/pnas.0506716102
id1402
pubmed_id16141315
bacterial resistance :
Ampicillin
cloning :
backbonepGL2 basic
backbone_mutation
backbone_originPromega
backbone_size5597
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
To generate pLDLR-Luc, a PCR fragment containing nucleotides 335 to +3 of the human LDL receptor gene was cloned into pGL2-basic. PCR'd using primers: CGATCCTCTACTGACCTC and GGCCCACGTCATTTACAGCATTTC.
origin :
37
pi :
alt_names
LDL receptor promoter
LDL receptor
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5SacI
promoter
sequencing_primer_3LucNrev
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFH, FHC, FHCL1, LDLCQ2
geneLDLR
id3949
genbank_ids
NC_000019
mutation
nameLDLR promoter
shRNA_sequence
size340
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagluciferase
resistance markers :
363
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA