This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV5 Flag-Smad2 EPSM
catalog :
14933
citations: 2
Reference
van den Akker G, van Beuningen H, Vitters E, Koenders M, van de Loo F, van Lent P, et al. Interleukin 1 β-induced SMAD2/3 linker modifications are TAK1 dependent and delay TGFβ signaling in primary human mesenchymal stem cells. Cell Signal. 2017;40:190-199 pubmed publisher
Kretzschmar M, Doody J, Timokhina I, Massague J. A mechanism of repression of TGFbeta/ Smad signaling by oncogenic Ras. Genes Dev. 1999;13:804-16 pubmed
product information
Catalog Number :
14933
Product Name :
pCMV5 Flag-Smad2 EPSM
article :
doi10.1101/gad.13.7.804
id1400
pubmed_id10197981
bacterial resistance :
Ampicillin
cloning :
backbonepCMV5
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
See article for more information.
origin :
37
pi :
alt_names
Smad2
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2
geneSMAD2
id4087
genbank_ids
mutationErk/Pro-directed kinase site mutant (EPSM). T220V, S245A, S250A, S255A.
nameSmad2 EPSM
shRNA_sequence
size1400
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
resistance markers :
313
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA