This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSG424 Smad2 (Gal4-Smad2)
catalog :
14932
citations: 1
Reference
Liu F, Pouponnot C, Massague J. Dual role of the Smad4/DPC4 tumor suppressor in TGFbeta-inducible transcriptional complexes. Genes Dev. 1997;11:3157-67 pubmed
product information
Catalog Number :
14932
Product Name :
pSG424 Smad2 (Gal4-Smad2)
article :
doi10.1101/gad.11.23.3157
id1399
pubmed_id9389648
bacterial resistance :
Ampicillin
cloning :
backbonepSG424
backbone_mutation
backbone_originMark Ptashne
backbone_size3400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
An EcoRI fragment of Human Smad2 encoding 21nts 5'UTR, the coding region of Smad2, and 50nts of 3'UTR was inserted into the EcoRI site of pSG424. The author's map shows the empty pSG424 map, which was described in Sadowski and Ptashne (1989) Nucleic Acids Research Vol 17 (18): p7539.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2
geneSMAD2
id4087
genbank_ids
AF027964
mutation
nameSmad2
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGal4
resistance markers :
313
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA