This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-sACE2v2-8his
catalog :
149270
citations: 2
Reference
Chan K, Dorosky D, Sharma P, Abbasi S, Dye J, Kranz D, et al. Engineering human ACE2 to optimize binding to the spike protein of SARS coronavirus 2. Science. 2020;369:1261-1265 pubmed publisher
Procko E. The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2. bioRxiv. 2020;: pubmed publisher
product information
Catalog Number :
149270
Product Name :
pcDNA3-sACE2v2-8his
article :
doi10.1126/science.abc0870
id28211097
pubmed_id32753553
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originInvitrogen
backbone_size5332
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
growth strain :
Mammalian expression plasmid for his-tagged sACE2 high affinity variant 2
origin :
37
pi :
alt_names
ACE2
peptidyl-dipeptidase A
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NheI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACEH
geneACE2
id59272
genbank_ids
NM_021804.1
mutationExtracellular, soluble protease domain (a.a. M1-D615) with mutations T27Y, L79T, N330Y, A386L
nameAngiotensin-converting enzyme 2
shRNA_sequence
size1902
species
9606
Homo sapiens
tags
locationC terminal on insert
tagShort GSG linker and 8his tag
resistance markers :
3492
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA