HLA-A2/K^b | Addgene 14906 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

HLA-A2/K^b

catalog :

14906

citations: 3

Reference
Mosaheb M, Dobrikova E, Brown M, Yang Y, Cable J, Okada H, et al. Genetically stable poliovirus vectors activate dendritic cells and prime antitumor CD8 T cell immunity. Nat Commun. 2020;11:524 pubmed publisher
Ding Z, Ou R, Ni B, Tang J, Xu Y. Cytolytic activity of the human papillomavirus type 16 E711-20 epitope-specific cytotoxic T lymphocyte is enhanced by heat shock protein 110 in HLA-A*0201 transgenic mice. Clin Vaccine Immunol. 2013;20:1027-33 pubmed publisher
Irwin M, Heath W, Sherman L. Species-restricted interactions between CD8 and the alpha 3 domain of class I influence the magnitude of the xenogeneic response. J Exp Med. 1989;170:1091-101 pubmed

product information

Catalog Number :

14906

Product Name :

HLA-A2/K^b

article :

doi	10.1084/jem.170.4.1091
id	1390
pubmed_id	2477484

bacterial resistance :

Ampicillin

cloning :

backbone

pSV2neo

backbone_mutation

backbone_origin

backbone_size

5700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

A Hind III-Bgl II fragment of the genomic clone of HLA-A2.1, which contained the genetic information coding for the leader sequence, alphal and alpha2 domains of the HLA-A2.1 molecule, was ligated to a Bam HI-Bam HI fragment excised from the Kb gene, which contained the alpha3, cytoplasmic, and transmembrane regions of the Kb molecule . The A2/Kb construct was purified and ligated into the Bam HI-Hind III sites of pSV2. The depositor notes that this contains the HLA-A2.1 Promoter, and was used for making the transgenic mouse in Vitiello A et.al. This construct was originally described as conferring Neomycin resistance, but based on user feedback, we are no longer confident that the Neo-resistance gene is intact.

origin :

pi :

alt_names

HLA-A2.1

H-2K^b

HLA-A

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	HindIII
promoter
sequencing_primer_3
sequencing_primer_5	SV40pro-F
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	HLAA
gene	HLA-A
id	3105

genbank_ids

mutation

name

HLA-A2/K^b

shRNA_sequence

size

8200

species

9606

Homo sapiens