YFP-DBD | Addgene 14874 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

YFP-DBD

catalog :

14874

citations: 2

Reference
Saheki Y, Bian X, Schauder C, Sawaki Y, Surma M, Klose C, et al. Control of plasma membrane lipid homeostasis by the extended synaptotagmins. Nat Cell Biol. 2016;18:504-15 pubmed publisher
Gallegos L, Kunkel M, Newton A. Targeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling. J Biol Chem. 2006;281:30947-56 pubmed

product information

Catalog Number :

14874

Product Name :

YFP-DBD

article :

doi	10.1074/jbc.M603741200
id	1377
pubmed_id	16901905

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

YFP-tagged DAG binding domain (YFP-DBD) was first constructed by fusion of monomeric YFP to the 5'-end of the C1b domain of PKCbeta. This construct was improved for DAG binding by introducing the Y123W point mutation via QuikChange (Stratagene).

origin :

pi :

alt_names

C1b-YFP

PKC beta II

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	HindIII
promoter
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Pkcb, Prkcb1
gene	Prkcb
id	25023

genbank_ids

mutation

Y123W

name

DAG binding domain

shRNA_sequence

size

species

10116

Rattus norvegicus