This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
DAGR
catalog :
14865
citations: 6
Reference
Renkhold L, Kollmann R, Inderwiedenstraße L, Kienitz M. PKC-isoform specific regulation of receptor desensitization and KCNQ1/KCNE1 K+ channel activity by mutant α1B-adrenergic receptors. Cell Signal. 2021;91:110228 pubmed publisher
da Silva Rosa S, Martens M, Field J, Nguyen L, Kereliuk S, Hai Y, et al. BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation. Autophagy. 2020;:1-16 pubmed publisher
Niemeyer A, Rinne A, Kienitz M. Receptor-specific regulation of atrial GIRK channel activity by different Ca2+-dependent PKC isoforms. Cell Signal. 2019;64:109418 pubmed publisher
Kienitz M, Vladimirova D, Muller C, Pott L, Rinne A. Receptor Species-dependent Desensitization Controls KCNQ1/KCNE1 K+ Channels as Downstream Effectors of Gq Protein-coupled Receptors. J Biol Chem. 2016;291:26410-26426 pubmed
Tauseef M, Knezevic N, Chava K, Smith M, Sukriti S, Gianaris N, et al. TLR4 activation of TRPC6-dependent calcium signaling mediates endotoxin-induced lung vascular permeability and inflammation. J Exp Med. 2012;209:1953-68 pubmed publisher
Violin J, Zhang J, Tsien R, Newton A. A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol. 2003;161:899-909 pubmed
product information
Catalog Number :
14865
Product Name :
DAGR
article :
doi10.1083/jcb.200302125
id1376
pubmed_id12782683
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
CFP and YFP fused to the C1A and C1B domains of PKCbetaII. DAGR was created by PCR of the C1A and C1B domains of rat PKC II (aa 37 152) from KpnI to XhoI and substitution for the PH domain in CYPHR.
origin :
37
pi :
alt_names
CFP-C1A-C1B-YFP
PKC beta II
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPkcb, Prkcb1
genePrkcb
id25023
genbank_ids
mutation
nameDAG reporter
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagCFP
locationC terminal on insert
tagYFP
resistance markers :
360
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA