CYPHR | Addgene 14864 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

CYPHR

catalog :

14864

citations: 2

Reference
Gildea J, Shah I, Van Sciver R, Israel J, Enzensperger C, McGrath H, et al. The cooperative roles of the dopamine receptors, D1R and D5R, on the regulation of renal sodium transport. Kidney Int. 2014;86:118-26 pubmed publisher
Violin J, Zhang J, Tsien R, Newton A. A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol. 2003;161:899-909 pubmed

product information

Catalog Number :

14864

Product Name :

CYPHR

article :

doi	10.1083/jcb.200302125
id	1376
pubmed_id	12782683

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

CFP and YFP fused to the PH domain of PLCdelta. CYPHR was generated by PCR of CFP from HindIII to KpnI, the PH domain of murine PLCdelta1 (aa 10 140) from KpnI to XhoI, and mYFP from XhoI to XbaI.

origin :

pi :

alt_names

CFP-PHdelta-YFP

PLC delta I

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	HindIII
promoter
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Plcd1
id	18799

genbank_ids

mutation

name

cyan/yellow PH domain reporter

shRNA_sequence

size

species