pJRL2 GAL1pr VYFP (EB#1571) | Addgene 14847 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pJRL2 GAL1pr VYFP (EB#1571)

catalog :

14847

citations: 1

Reference
Raser J, O Shea E. Control of stochasticity in eukaryotic gene expression. Science. 2004;304:1811-4 pubmed

product information

Catalog Number :

14847

Product Name :

pJRL2 GAL1pr VYFP (EB#1571)

article :

doi	10.1126/science.1098641
id	1373
pubmed_id	15166317

bacterial resistance :

Ampicillin

cloning :

backbone

pJRL2

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

pJRL2 is a yeast chromosomal integration vector that replaces the LEU2 chromosomal locus by homologous recombination. pJRL2 was constructed by insertion of the 200 bp upstream of the LEU2 start codon, an Asc I restriction enzyme site, the 200 bp downstream of the LEU2 stop codon and a 5.5 kb hisG::URA3::kanR::hisG cassette into pBluescript. The contruct contains 1000 bp of promoter sequence, from the nucleotide immediately upstream of the start codon, followed by six base-pairs of idealized Kozak sequence AACAAA for optimal translational initiation, the coding sequence of the appropriate fluorophore (yECFP or yVYFP) and the 500 bp 3 to the stop codon of ACT1 in the pJRL2 integrating vector. The GAL1 promoter was amplified from genomic DNA.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YBR020W
gene	GAL1
id	852308

genbank_ids

mutation

name

GAL1 promoter

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae