pRK5 TGF beta type I receptor (T202D) Flag | Addgene 14833 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRK5 TGF beta type I receptor (T202D) Flag

catalog :

14833

citations: 3

Reference
Melendez J, Sieiro D, Salgado D, Morin V, Dejardin M, Zhou C, et al. TGFβ signalling acts as a molecular brake of myoblast fusion. Nat Commun. 2021;12:749 pubmed publisher
Chen C, Kao Y, Yang P, Sung P, Wen Z, Chen J, et al. A Small Dibromotyrosine Derivative Purified From Pseudoceratina Sp. Suppresses TGF-Î² Responsiveness by Inhibiting TGF-Î² Type I Receptor Serine/Threonine Kinase Activity. J Cell Biochem. 2016;117:2800-2814 pubmed publisher
Feng X, Derynck R. Ligand-independent activation of transforming growth factor (TGF) beta signaling pathways by heteromeric cytoplasmic domains of TGF-beta receptors. J Biol Chem. 1996;271:13123-9 pubmed

product information

Catalog Number :

14833

Product Name :

pRK5 TGF beta type I receptor (T202D) Flag

article :

doi	10.1074/jbc.271.22.13123
id	1370
pubmed_id	8662796

bacterial resistance :

Ampicillin

cloning :

backbone

pRK5 modified with flag

backbone_mutation

backbone_origin

Genentech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

See Author's map for more information. A 9bp linker was added directly upstream of the GS domain. The linker encodes the amino acids GSM. The additional sequence does not affect plasmid function.

origin :

pi :

alt_names

TGFb type I receptor

Tgfbr1

ALK5

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	EcoRI
promoter
sequencing_primer_3
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Alk5, Skr4, Tgfr-1, tbetaR-I
gene	Tgfbr1
id	29591

genbank_ids

mutation

Threonine 202 mutated to aspartate. Constitutively active mutant.

name

TGF beta type I receptor

shRNA_sequence

size

species

10116

Rattus norvegicus