This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRK5 TGF beta type I receptor Flag
catalog :
14831
citations: 3
Reference
Murillo Garzón V, Gorroño Etxebarria I, Akerfelt M, Puustinen M, Sistonen L, Nees M, et al. Frizzled-8 integrates Wnt-11 and transforming growth factor-β signaling in prostate cancer. Nat Commun. 2018;9:1747 pubmed publisher
Yamanaka O, Yuan Y, Coulson Thomas V, Gesteira T, Call M, Zhang Y, et al. Lumican binds ALK5 to promote epithelium wound healing. PLoS ONE. 2013;8:e82730 pubmed publisher
Feng X, Derynck R. Ligand-independent activation of transforming growth factor (TGF) beta signaling pathways by heteromeric cytoplasmic domains of TGF-beta receptors. J Biol Chem. 1996;271:13123-9 pubmed
product information
Catalog Number :
14831
Product Name :
pRK5 TGF beta type I receptor Flag
article :
doi10.1074/jbc.271.22.13123
id1370
pubmed_id8662796
bacterial resistance :
Ampicillin
cloning :
backbonepRK5 modified with flag
backbone_mutation
backbone_originGenentech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
See Author's map for more information. A 9bp linker was added directly upstream of the GS domain. The linker encodes the amino acids GSM. The additional sequence does not affect plasmid function.
origin :
37
pi :
alt_names
TGFb type I receptor
Tgfbr1
ALK5
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoter
sequencing_primer_3SV40pA-R
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAlk5, Skr4, Tgfr-1, tbetaR-I
geneTgfbr1
id29591
genbank_ids
mutation
nameTGF beta type I receptor
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationC terminal on backbone
tagflag
resistance markers :
115
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA