pRD119 (PCNA GFP) | Addgene 14822 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRD119 (PCNA GFP)

catalog :

14822

citations: 1

Reference
Thacker S, Bonnette P, Duronio R. The contribution of E2F-regulated transcription to Drosophila PCNA gene function. Curr Biol. 2003;13:53-8 pubmed

product information

Catalog Number :

14822

Product Name :

pRD119 (PCNA GFP)

article :

doi	10.1016/S0960-9822(02)01400-8
id	1366
pubmed_id	12526745

bacterial resistance :

Ampicillin

cloning :

backbone

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

PCNA GFP fusion (glow); Asp718-NotI fragment from pRD113 cloned into pCasper4IN; GFP fused in-frame using 5' blunt NheI and 3' XbaI (replaces PCNA coding to STOP [XbaI] but has PCNA 3'; GFP from pNEGFPX.1.

origin :

pi :

alt_names

PCNA

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	na
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG9193, 53/13, CG9193, DmPCNA, DmPCNA1, Dmel CG9193, MUS209, Mus209, PCNA/mus209, PCNA1, Pcna, dPCNA, l(2)02448, l(2)56F, l(2)56Fa, l(2)s1534, mus 209, mus209, pcna
gene	PCNA
id	37290

genbank_ids

mutation

name

PCNA-GFP

shRNA_sequence

size

species

7227

Drosophila melanogaster