This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCAG-CreERT2
catalog :
14797
citations: 40
Reference
Fujimoto S, Leiwe M, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, et al. Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Dev Cell. 2023;58:1221-1236.e7 pubmed publisher
Sikder K, Phillips E, Zhong Z, Wang N, Saunders J, Mothy D, et al. Perinuclear damage from nuclear envelope deterioration elicits stress responses that contribute to LMNA cardiomyopathy. bioRxiv. 2023;: pubmed publisher
Travnickova J, Muise S, Wojciechowska S, Brombin A, Zeng Z, Young A, et al. Fate mapping melanoma persister cells through regression and into recurrent disease in adult zebrafish. Dis Model Mech. 2022;15: pubmed publisher
Lin C, Sun Y, Chan C, Wu M, Gu L, Davis A, et al. Identification of cis-regulatory modules for adeno-associated virus-based cell-type-specific targeting in the retina and brain. J Biol Chem. 2022;298:101674 pubmed publisher
Fabra Beser J, Alves Medeiros de Araujo J, Marques Coelho D, Goff L, Costa M, Muller U, et al. Differential expression levels of Sox9 in early neocortical radial glial cells regulate the decision between stem cell maintenance and differentiation. J Neurosci. 2021;: pubmed publisher
Kuwano T, Izumi H, Aslam M, Igarashi Y, Bilal M, Nishimura A, et al. Generation and characterization of a Meflin-CreERT2 transgenic line for lineage tracing in white adipose tissue. PLoS ONE. 2021;16:e0248267 pubmed publisher
Kaczmarczyk L, Reichenbach N, Blank N, Jonson M, Dittrich L, Petzold G, et al. Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background. Sci Rep. 2021;11:5412 pubmed publisher
Castellan M, Guarnieri A, Fujimura A, Zanconato F, Battilana G, Panciera T, et al. Single-cell analyses reveal YAP/TAZ as regulators of stemness and cell plasticity in Glioblastoma. Nat Cancer. 2021;2:174-188 pubmed publisher
Ichijo R, Kabata M, Kidoya H, Muramatsu F, Ishibashi R, Abe K, et al. Vasculature-driven stem cell population coordinates tissue scaling in dynamic organs. Sci Adv. 2021;7: pubmed publisher
Yamagata M, Yan W, Sanes J. A cell atlas of the chick retina based on single-cell transcriptomics. elife. 2021;10: pubmed publisher
Ishimoto R, Tsuzuki Y, Matsumura T, Kurashige S, Enokitani K, Narimatsu K, et al. SLX4-XPF mediates DNA damage responses to replication stress induced by DNA-protein interactions. J Cell Biol. 2021;220: pubmed publisher
Zhang M, Lai Y, Krupalnik V, Guo P, Guo X, Zhou J, et al. β-Catenin safeguards the ground state of mousepluripotency by strengthening the robustness of the transcriptional apparatus. Sci Adv. 2020;6:eaba1593 pubmed publisher
Sánchez González R, Bribian A, López Mascaraque L. Cell Fate Potential of NG2 Progenitors. Sci Rep. 2020;10:9876 pubmed publisher
Morikawa K, Furuhashi K, de Sena Tomás C, Garcia Garcia A, Bekdash R, Klein A, et al. Photoactivatable Cre recombinase 3.0 for in vivo mouse applications. Nat Commun. 2020;11:2141 pubmed publisher
Scott R, Arostegui M, Schweitzer R, Rossi F, Underhill T. Hic1 Defines Quiescent Mesenchymal Progenitor Subpopulations with Distinct Functions and Fates in Skeletal Muscle Regeneration. Cell Stem Cell. 2019;25:797-813.e9 pubmed publisher
Allen M, Zhou W, Thangaraj J, Kyriakakis P, Wu Y, Huang Z, et al. An AND-Gated Drug and Photoactivatable Cre-loxP System for Spatiotemporal Control in Cell-Based Therapeutics. ACS Synth Biol. 2019;8:2359-2371 pubmed publisher
Rocha Martins M, de Toledo B, Santos França P, Oliveira Valença V, Vieira Vieira C, Matos Rodrigues G, et al. De novo genesis of retinal ganglion cells by targeted expression of Klf4 in vivo. Development. 2019;146: pubmed publisher
Kallunki T, Barisic M, Jaattela M, Liu B. How to Choose the Right Inducible Gene Expression System for Mammalian Studies?. Cells. 2019;8: pubmed publisher
Wu J, Kung J, Dong J, Chang L, Xie C, Habib A, et al. Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning. Cell Rep. 2019;28:1167-1181.e7 pubmed publisher
Pineault K, Song J, Kozloff K, Lucas D, Wellik D. Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life. Nat Commun. 2019;10:3168 pubmed publisher
Sakaguchi R, Leiwe M, Imai T. Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags. elife. 2018;7: pubmed publisher
Chen Z, Ren X, Xu X, Zhang X, Hui Y, Liu Z, et al. Genetic Engineering of Human Embryonic Stem Cells for Precise Cell Fate Tracing during Human Lineage Development. Stem Cell Reports. 2018;11:1257-1271 pubmed publisher
Tabor K, Smith T, Brown M, Bergeron S, Briggman K, Burgess H. Presynaptic Inhibition Selectively Gates Auditory Transmission to the Brainstem Startle Circuit. Curr Biol. 2018;28:2527-2535.e8 pubmed publisher
Yoshimura Y, Ida Tanaka M, Hiramaki T, Goto M, Kamisako T, Eto T, et al. Novel reporter and deleter mouse strains generated using VCre/VloxP and SCre/SloxP systems, and their system specificity in mice. Transgenic Res. 2018;27:193-201 pubmed publisher
Takebe T, Sakamoto K, Higami Y, Harada Y. A novel mouse model for tracking the fate of CXCR5-expressing T cells. Biochem Biophys Res Commun. 2018;495:1642-1647 pubmed publisher
Doi T, Ogata T, Yamauchi J, Sawada Y, Tanaka S, Nagao M. Chd7 Collaborates with Sox2 to Regulate Activation of Oligodendrocyte Precursor Cells after Spinal Cord Injury. J Neurosci. 2017;37:10290-10309 pubmed publisher
Shiota C, Prasadan K, Guo P, Fusco J, Xiao X, Gittes G. Gcg CreERT2 knockin mice as a tool for genetic manipulation in pancreatic alpha cells. Diabetologia. 2017;60:2399-2408 pubmed publisher
Nawaz A, Aminuddin A, Kado T, Takikawa A, Yamamoto S, Tsuneyama K, et al. CD206+ M2-like macrophages regulate systemic glucose metabolism by inhibiting proliferation of adipocyte progenitors. Nat Commun. 2017;8:286 pubmed publisher
Decker R, Um H, Dyment N, Cottingham N, Usami Y, Enomoto Iwamoto M, et al. Cell origin, volume and arrangement are drivers of articular cartilage formation, morphogenesis and response to injury in mouse limbs. Dev Biol. 2017;426:56-68 pubmed publisher
Xu J, Yao G, Ru Y, Xie S. Expression of tamoxifen-inducible CRE recombinase in Lcn5-CreERT2 transgenic mouse caput epididymis. Mol Reprod Dev. 2017;84:257-264 pubmed publisher
Figueres Oñate M, García Marqués J, López Mascaraque L. UbC-StarTrack, a clonal method to target the entire progeny of individual progenitors. Sci Rep. 2016;6:33896 pubmed publisher
Gao D, Zhan Y, Di W, Moore A, Sher J, Guan Y, et al. A Tmprss2-CreERT2 Knock-In Mouse Model for Cancer Genetic Studies on Prostate and Colon. PLoS ONE. 2016;11:e0161084 pubmed publisher
Feng J, Jing J, Sanchez Lara P, Bootwalla M, Buckley J, Wu N, et al. Generation and characterization of tamoxifen-inducible Pax9-CreER knock-in mice using CrispR/Cas9. Genesis. 2016;54:490-6 pubmed publisher
Evaristo C, Spranger S, Barnes S, Miller M, Molinero L, Locke F, et al. Cutting Edge: Engineering Active IKKβ in T Cells Drives Tumor Rejection. J Immunol. 2016;196:2933-8 pubmed publisher
Hofstetter C, Kampka J, Huppertz S, Weber H, Schlosser A, Müller A, et al. Inhibition of KDM6 activity during murine ESC differentiation induces DNA damage. J Cell Sci. 2016;129:788-803 pubmed publisher
Yuan L, Chan G, Beeler D, Janes L, Spokes K, Dharaneeswaran H, et al. A role of stochastic phenotype switching in generating mosaic endothelial cell heterogeneity. Nat Commun. 2016;7:10160 pubmed publisher
Horstick E, Jordan D, Bergeron S, Tabor K, Serpe M, Feldman B, et al. Increased functional protein expression using nucleotide sequence features enriched in highly expressed genes in zebrafish. Nucleic Acids Res. 2015;43:e48 pubmed publisher
Rudat C, Grieskamp T, Röhr C, Airik R, Wrede C, Hegermann J, et al. Upk3b is dispensable for development and integrity of urothelium and mesothelium. PLoS ONE. 2014;9:e112112 pubmed publisher
Pak T, Yoo S, Miranda Angulo A, Miranda Angulo A, Wang H, Blackshaw S. Rax-CreERT2 knock-in mice: a tool for selective and conditional gene deletion in progenitor cells and radial glia of the retina and hypothalamus. PLoS ONE. 2014;9:e90381 pubmed publisher
Matsuda T, Cepko C. Controlled expression of transgenes introduced by in vivo electroporation. Proc Natl Acad Sci U S A. 2007;104:1027-32 pubmed
product information
Catalog Number :
14797
Product Name :
pCAG-CreERT2
article :
doi10.1073/pnas.0610155104
id1096
pubmed_id17209010
bacterial resistance :
Ampicillin
cloning :
backbonepCAGEN
backbone_mutation
backbone_origin
backbone_size4779
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Cre/Lox
growth notes :
4-hydroxitamoxifen-responsible Cre. Kozak consensus sequence was added before the start ATG. The insert can be cut out with EcoRI and NotI.
growth strain :
Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)
origin :
37
pi :
alt_names
inducible Cre
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5pCAG-F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCre-ERT2 fusion protein
shRNA_sequence
size2004
species
Bacteriophage P1
tags
plasmid copy :
CreERT2 was modified from pCreERT2 (Feil et al. BBRC, 237, 752-757 (1997) obtained from Dr. P. Chambon (Universite Louis Pasteur).
resistance markers :
71
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA