pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-CG32016-E_T | Addgene 147588 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-CG32016-E_T

catalog :

147588

citations: 1

Reference
Igreja C, Peter D, Weiler C, Izaurralde E. 4E-BPs require non-canonical 4E-binding motifs and a lateral surface of eIF4E to repress translation. Nat Commun. 2014;5:4790 pubmed publisher

product information

Catalog Number :

147588

Product Name :

pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-CG32016-E_T

article :

doi	10.1038/ncomms5790
id	18593
pubmed_id	25179781

bacterial resistance :

Ampicillin

cloning :

backbone

Unknown

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

DmEIF4G ORF extracted from NCBI: NM_079901.3. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact help@addgene.org before placing your order to request that our lab fully sequence the plasmid.

growth strain :

Insect Expression of DmeIF4Ga_578-650-CG32016-E

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG10811, CG10811, Dmel CG10811, EIF4G, Eif4G, deIF4G, eIF-4G, eIF4G, eIF4g, p200
gene	eIF4G1
id	43839

genbank_ids

mutation

CG32016-E 5 silent and three no silent mutations H117R, N148S and P315H compared to the sequence given by NCBI:NM_166800.1

name

DmeIF4Ga_578-650-CG32016-E

shRNA_sequence

size

species

7227

Drosophila melanogaster