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product summary
company name :
Addgene
product type :
cDNA
product name :
HA GSK3 beta S9A pcDNA3
catalog :
14754
citations: 50
Reference
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Cook B, Walker N, Zhang Q, Chen S, Evans T. The small molecule DIPQUO promotes osteogenic differentiation via inhibition of glycogen synthase kinase 3-beta signaling. J Biol Chem. 2021;296:100696 pubmed publisher
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Sun H, Zhu A, Gao Y, Terajima H, Fei Q, Liu S, et al. Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m6A Methylation. Mol Cell. 2020;80:633-647.e7 pubmed publisher
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Hou B, Li Y, Li X, Zhang C, Zhao Z, Chen Q, et al. HGF protected against diabetic nephropathy via autophagy-lysosome pathway in podocyte by modulating PI3K/Akt-GSK3β-TFEB axis. Cell Signal. 2020;75:109744 pubmed publisher
Ragu Varman D, Jayanthi L, Ramamoorthy S. Glycogen synthase kinase-3ß supports serotonin transporter function and trafficking in a phosphorylation-dependent manner. J Neurochem. 2020;: pubmed publisher
Zhang B, Li Z, Zhang R, Hu Y, Jiang Y, Cao T, et al. PKCγ promotes axonal remodeling in the cortico-spinal tract via GSK3β/β-catenin signaling after traumatic brain injury. Sci Rep. 2019;9:17078 pubmed publisher
Gao J, Long L, Xu F, Feng L, Liu Y, Shi J, et al. Icariside II, a phosphodiesterase 5 inhibitor, attenuates cerebral ischemia/reperfusion injury by inhibiting GSK-3β-mediated activation of autophagy. Br J Pharmacol. 2019;: pubmed publisher
Li D, Cho Y. High specificity of widely used phospho-tau antibodies validated using a quantitative whole-cell based assay. J Neurochem. 2020;152:122-135 pubmed publisher
Singh R, Holz P, Roth K, Hupfer A, Meissner W, Muller R, et al. DYRK1B regulates Hedgehog-induced microtubule acetylation. Cell Mol Life Sci. 2019;76:193-207 pubmed publisher
Pei S, Minhajuddin M, Adane B, Khan N, Stevens B, Mack S, et al. AMPK/FIS1-Mediated Mitophagy Is Required for Self-Renewal of Human AML Stem Cells. Cell Stem Cell. 2018;23:86-100.e6 pubmed publisher
Martin S, Souder D, Miller K, Clark J, Sagar A, Eliceiri K, et al. GSK3? Regulates Brain Energy Metabolism. Cell Rep. 2018;23:1922-1931.e4 pubmed publisher
Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed publisher
Markussen L, Winther S, Wicksteed B, Hansen J. GSK3 is a negative regulator of the thermogenic program in brown adipocytes. Sci Rep. 2018;8:3469 pubmed publisher
Tong Y, Park S, Wu D, Harris T, Moskaluk C, Brautigan D, et al. Modulation of GSK3β autoinhibition by Thr-7 and Thr-8. FEBS Lett. 2018;592:537-546 pubmed publisher
Abd Ellah A, Voogdt C, Krappmann D, Moller P, Marienfeld R. GSK3β modulates NF-κB activation and RelB degradation through site-specific phosphorylation of BCL10. Sci Rep. 2018;8:1352 pubmed publisher
Fei Y, Xiong Y, Shen X, Zhao Y, Zhu Y, Wang L, et al. Cathepsin L promotes ionizing radiation-induced U251 glioma cell migration and invasion through regulating the GSK-3β/CUX1 pathway. Cell Signal. 2018;44:62-71 pubmed publisher
Gozdz A, Nikolaienko O, Urbanska M, Cymerman I, Sitkiewicz E, Blazejczyk M, et al. GSK3? and GSK3? Phosphorylate Arc and Regulate its Degradation. Front Mol Neurosci. 2017;10:192 pubmed publisher
Gao S, Li S, Duan X, Gu Z, Ma Z, Yuan X, et al. Inhibition of glycogen synthase kinase 3 beta (GSK3β) suppresses the progression of esophageal squamous cell carcinoma by modifying STAT3 activity. Mol Carcinog. 2017;56:2301-2316 pubmed publisher
Durán J, Oyarce C, Pávez M, Valladares D, Basualto Alarcón C, Lagos D, et al. GSK-3?/NFAT Signaling Is Involved in Testosterone-Induced Cardiac Myocyte Hypertrophy. PLoS ONE. 2016;11:e0168255 pubmed publisher
Gupta P, Srivastav S, Saha S, Das P, Ukil A. Leishmania donovani inhibits macrophage apoptosis and pro-inflammatory response through AKT-mediated regulation of β-catenin and FOXO-1. Cell Death Differ. 2016;23:1815-1826 pubmed publisher
Hong X, Liu W, Song R, Shah J, Feng X, Tsang C, et al. SOX9 is targeted for proteasomal degradation by the E3 ligase FBW7 in response to DNA damage. Nucleic Acids Res. 2016;44:8855-8869 pubmed
Wu Q. Serpine2, a potential novel target for combating melanoma metastasis. Am J Transl Res. 2016;8:1985-97 pubmed
Tao L, Sheng X, Zhang L, Li W, Wei Z, Zhu P, et al. Xanthatin anti-tumor cytotoxicity is mediated via glycogen synthase kinase-3? and ?-catenin. Biochem Pharmacol. 2016;115:18-27 pubmed publisher
Miao L, Yang L, Huang H, Liang F, Ling C, Hu Y. mTORC1 is necessary but mTORC2 and GSK3? are inhibitory for AKT3-induced axon regeneration in the central nervous system. elife. 2016;5:e14908 pubmed publisher
Gu Z, Lamont G, Lamont R, Uriarte S, Wang H, Scott D. Resolvin D1, resolvin D2 and maresin 1 activate the GSK3β anti-inflammatory axis in TLR4-engaged human monocytes. Innate Immun. 2016;22:186-95 pubmed publisher
Bai L, Yu Z, Zhang J, Yuan S, Liao C, Jeyabal P, et al. OLA1 contributes to epithelial-mesenchymal transition in lung cancer by modulating the GSK3β/snail/E-cadherin signaling. Oncotarget. 2016;7:10402-13 pubmed publisher
Ngkelo A, Hoffmann R, Durham A, Marwick J, Brandenburg S, de Bruin H, et al. Glycogen synthase kinase-3β modulation of glucocorticoid responsiveness in COPD. Am J Physiol Lung Cell Mol Physiol. 2015;309:L1112-23 pubmed publisher
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Tao L, Fan F, Liu Y, Li W, Zhang L, Ruan J, et al. Concerted suppression of STAT3 and GSK3? is involved in growth inhibition of non-small cell lung cancer by Xanthatin. PLoS ONE. 2013;8:e81945 pubmed publisher
Yin Y, Zhang X, Li Z, Deng L, Jiao G, Zhang B, et al. Glucocorticoid receptor ? regulates injury-mediated astrocyte activation and contributes to glioma pathogenesis via modulation of ?-catenin/TCF transcriptional activity. Neurobiol Dis. 2013;59:165-76 pubmed publisher
Darr J, Klochendler A, Isaac S, Eden A. Loss of IGFBP7 expression and persistent AKT activation contribute to SMARCB1/Snf5-mediated tumorigenesis. Oncogene. 2014;33:3024-32 pubmed publisher
Ramser E, Gan K, Decker H, Fan E, Suzuki M, Ferreira S, et al. Amyloid-? oligomers induce tau-independent disruption of BDNF axonal transport via calcineurin activation in cultured hippocampal neurons. Mol Biol Cell. 2013;24:2494-505 pubmed publisher
Patel R, Gao M, Ahmad I, Fleming J, Singh L, Rai T, et al. Sprouty2, PTEN, and PP2A interact to regulate prostate cancer progression. J Clin Invest. 2013;123:1157-75 pubmed publisher
Benseddik K, Sen Nkwe N, Daou P, Verdier Pinard P, Badache A. ErbB2-dependent chemotaxis requires microtubule capture and stabilization coordinated by distinct signaling pathways. PLoS ONE. 2013;8:e55211 pubmed publisher
Frank C, Reguerio V, Rother M, Moranta D, Maeurer A, Garmendia J, et al. Klebsiella pneumoniae targets an EGF receptor-dependent pathway to subvert inflammation. Cell Microbiol. 2013;15:1212-33 pubmed publisher
Cozzolino A, Alonzi T, Santangelo L, Mancone C, Conti B, Steindler C, et al. TGF? overrides HNF4? tumor suppressing activity through GSK3? inactivation: implication for hepatocellular carcinoma gene therapy. J Hepatol. 2013;58:65-72 pubmed publisher
Martin V, Wang W, Lipchik A, Parker L, He Y, Zhang S, et al. Akt2 inhibits the activation of NFAT in lymphocytes by modulating calcium release from intracellular stores. Cell Signal. 2012;24:1064-73 pubmed publisher
Saint Just Ribeiro M, Hansson M, Lindberg M, Popko Scibor A, Wallberg A. GSK3beta is a negative regulator of the transcriptional coactivator MAML1. Nucleic Acids Res. 2009;37:6691-700 pubmed publisher
Stambolic V, Woodgett J. Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation. Biochem J. 1994;303 ( Pt 3):701-4 pubmed
product information
Catalog Number :
14754
Product Name :
HA GSK3 beta S9A pcDNA3
article :
doi
id1353
pubmed_id7980435
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
S2A in the sequence does not affect function, used to introduce restriction enzyme site
growth strain :
Mammalian expression of human GSK3 beta constitutively active mutant with HA tag
origin :
37
pi :
alt_names
GSK3b
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneGSK3B
id2932
genbank_ids
mutationS9A. Constitutively active mutant.
nameGSK3 beta S9A
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHA
resistance markers :
586
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA