This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HA PKB T308D S473D pcDNA3
catalog :
14751
citations: 17
Reference
Wu Q, Tian A, Li B, Leduc M, Forveille S, Hamley P, et al. IGF1 receptor inhibition amplifies the effects of cancer drugs by autophagy and immune-dependent mechanisms. J Immunother Cancer. 2021;9: pubmed publisher
Chen X, He L, Lai S, He Y. Dihydroartemisinin inhibits the migration of esophageal cancer cells by inducing autophagy. Oncol Lett. 2020;20:94 pubmed publisher
Tian S, Lou L, Tian M, Lu G, Tian J, Chen X. MAPK4 deletion enhances radiation effects and triggers synergistic lethality with simultaneous PARP1 inhibition in cervical cancer. J Exp Clin Cancer Res. 2020;39:143 pubmed publisher
Kim J, Han N, Park S, Jegal K, Jung J, Jung E, et al. Hemistepsin A alleviates liver fibrosis by inducing apoptosis of activated hepatic stellate cells via inhibition of nuclear factor-κB and Akt. Food Chem Toxicol. 2020;135:111044 pubmed publisher
Liu S, Yuan D, Li Y, Qi Q, Guo B, Yang S, et al. Involvement of Phosphatase and Tensin Homolog in Cyclin-Dependent Kinase 4/6 Inhibitor-Induced Blockade of Glioblastoma. Front Pharmacol. 2019;10:1316 pubmed publisher
Shan L, Liu W, Zhan Y. Sulfated polysaccharide of Sepiella maindroni ink targets Akt and overcomes resistance to the FGFR inhibitor AZD4547 in bladder cancer. Aging (Albany NY). 2019;11:7780-7795 pubmed publisher
Maiuthed A, Ninsontia C, Erlenbach Wuensch K, Ndreshkjana B, Muenzner J, Caliskan A, et al. Cytoplasmic p21 Mediates 5-Fluorouracil Resistance by Inhibiting Pro-Apoptotic Chk2. Cancers (Basel). 2018;10: pubmed publisher
Lin R, Xia S, Shan C, Chen D, Liu Y, Gao X, et al. The Dietary Supplement Chondroitin-4-Sulfate Exhibits Oncogene-Specific Pro-tumor Effects on BRAF V600E Melanoma Cells. Mol Cell. 2018;69:923-937.e8 pubmed publisher
Liu J, Quan L, Zhang C, Liu A, Tong D, Wang J. Over-activated PD-1/PD-L1 axis facilitates the chemoresistance of diffuse large B-cell lymphoma cells to the CHOP regimen. Oncol Lett. 2018;15:3321-3328 pubmed publisher
Zubair H, Azim S, Srivastava S, Ahmad A, Bhardwaj A, Khan M, et al. Glucose Metabolism Reprogrammed by Overexpression of IKKε Promotes Pancreatic Tumor Growth. Cancer Res. 2016;76:7254-7264 pubmed
Cai X, Chen L, Song H, Zhang C, Yuan Z, Xiang Z. PLK1 promotes epithelial-mesenchymal transition and metastasis of gastric carcinoma cells. Am J Transl Res. 2016;8:4172-4183 pubmed
Makhov P, Golovine K, Teper E, Kutikov A, Mehrazin R, Corcoran A, et al. Piperlongumine promotes autophagy via inhibition of Akt/mTOR signalling and mediates cancer cell death. Br J Cancer. 2014;110:899-907 pubmed publisher
Belal A, Sell B, Hoi H, Davidson M, Campbell R. Optimization of a genetically encoded biosensor for cyclin B1-cyclin dependent kinase 1. Mol Biosyst. 2014;10:191-5 pubmed publisher
Hung S, Shih Y, Chen M, Lo S. Up-regulated cten by FGF2 contributes to FGF2-mediated cell migration. Mol Carcinog. 2014;53:787-92 pubmed publisher
Imam J, Plyler J, Bansal H, Prajapati S, Bansal S, Rebeles J, et al. Genomic loss of tumor suppressor miRNA-204 promotes cancer cell migration and invasion by activating AKT/mTOR/Rac1 signaling and actin reorganization. PLoS ONE. 2012;7:e52397 pubmed publisher
Martin V, Wang W, Lipchik A, Parker L, He Y, Zhang S, et al. Akt2 inhibits the activation of NFAT in lymphocytes by modulating calcium release from intracellular stores. Cell Signal. 2012;24:1064-73 pubmed publisher
Scheid M, Marignani P, Woodgett J. Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B. Mol Cell Biol. 2002;22:6247-60 pubmed
product information
Catalog Number :
14751
Product Name :
HA PKB T308D S473D pcDNA3
article :
doi10.1128/MCB.22.17.6247-6260.2002
id1348
pubmed_id12167717
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
KpnI(1934) and NheI(2429) represent T308->D and S473->D mutations respectively
origin :
37
pi :
alt_names
Protein Kinase B alpha
Akt1
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBOS_20453
geneAKT1
id280991
genbank_ids
mutationT308D S473D. Constitutively active mutant.
namePKB DD
shRNA_sequence
size1500
species
9913
Bos taurus
tags
locationN terminal on insert
tagHA
resistance markers :
586
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA