This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MEK1-GFP
catalog :
14746
citations: 15
Reference
Yang Q, Li F, Jia G, Liu R. Aged black garlic extract inhibits the growth of estrogen receptor-positive breast cancer cells by downregulating MCL-1 expression through the ROS-JNK pathway. PLoS ONE. 2023;18:e0286454 pubmed publisher
Yang H, Gao Y, Xu D, Xu K, Liang S, Yang Z, et al. MEK1 drives oncogenic signaling and interacts with PARP1 for genomic and metabolic homeostasis in malignant pleural mesothelioma. Cell Death Discov. 2023;9:55 pubmed publisher
Hanrahan A, SYLVESTER B, Chang M, Elzein A, Gao J, Han W, et al. Leveraging systematic functional analysis to benchmark an in silico framework distinguishes driver from passenger MEK mutants in cancer. Cancer Res. 2020;: pubmed publisher
Chao M, Chang L, Tu H, Chang C, Lai M, Chen Y, et al. Combination treatment strategy for pancreatic cancer involving the novel HDAC inhibitor MPT0E028 with a MEK inhibitor beyond K-Ras status. Clin Epigenetics. 2019;11:85 pubmed publisher
Bielski C, Donoghue M, Gadiya M, Hanrahan A, Won H, Chang M, et al. Widespread Selection for Oncogenic Mutant Allele Imbalance in Cancer. Cancer Cell. 2018;34:852-862.e4 pubmed publisher
Azorsa D, Lee D, Wai D, Bista R, Patel A, Aleem E, et al. Clinical resistance associated with a novel MAP2K1 mutation in a patient with Langerhans cell histiocytosis. Pediatr Blood Cancer. 2018;65:e27237 pubmed publisher
Mita P, Wudzinska A, Sun X, Andrade J, Nayak S, Kahler D, et al. LINE-1 protein localization and functional dynamics during the cell cycle. elife. 2018;7: pubmed publisher
Li W, Reeb A, Lin B, Subramanian P, Fey E, Knoverek C, et al. Heat Shock-induced Phosphorylation of TAR DNA-binding Protein 43 (TDP-43) by MAPK/ERK Kinase Regulates TDP-43 Function. J Biol Chem. 2017;292:5089-5100 pubmed publisher
Gao J, Chang M, Johnsen H, Gao S, SYLVESTER B, Sumer S, et al. 3D clusters of somatic mutations in cancer reveal numerous rare mutations as functional targets. Genome Med. 2017;9:4 pubmed publisher
Dhawan N, Scopton A, Dar A. Small molecule stabilization of the KSR inactive state antagonizes oncogenic Ras signalling. Nature. 2016;537:112-116 pubmed publisher
Arcila M, Drilon A, SYLVESTER B, Lovly C, Borsu L, Reva B, et al. MAP2K1 (MEK1) Mutations Define a Distinct Subset of Lung Adenocarcinoma Associated with Smoking. Clin Cancer Res. 2015;21:1935-43 pubmed publisher
Chakraborty R, Hampton O, Shen X, Simko S, Shih A, Abhyankar H, et al. Mutually exclusive recurrent somatic mutations in MAP2K1 and BRAF support a central role for ERK activation in LCH pathogenesis. Blood. 2014;124:3007-15 pubmed publisher
Brannon A, Vakiani E, SYLVESTER B, Scott S, McDermott G, Shah R, et al. Comparative sequencing analysis reveals high genomic concordance between matched primary and metastatic colorectal cancer lesions. Genome Biol. 2014;15:454 pubmed publisher
Peng H, Dara L, Li T, Zheng Y, Yang H, Tomasi M, et al. MAT2B-GIT1 interplay activates MEK1/ERK 1 and 2 to induce growth in human liver and colon cancer. Hepatology. 2013;57:2299-313 pubmed publisher
Yao Z, Flash I, Raviv Z, Yung Y, Asscher Y, Pleban S, et al. Non-regulated and stimulated mechanisms cooperate in the nuclear accumulation of MEK1. Oncogene. 2001;20:7588-96 pubmed
product information
Catalog Number :
14746
Product Name :
MEK1-GFP
article :
doi10.1038/sj.onc.1204963
id1344
pubmed_id11753637
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid grows slowly. We encourage requesting scientists to allow the plasmid to grow for a longer period of time than normal.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5HindIII
promoter
sequencing_primer_3EGFP-N
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1
geneMAP2K1
id5604
genbank_ids
mutation
nameMEK1
shRNA_sequence
size1200
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagEGFP
resistance markers :
454
tags :
Low Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA