pAc5.1B-lambdaN-HA-DmNot4-dsRNAres_Q | Addgene 147340 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAc5.1B-lambdaN-HA-DmNot4-dsRNAres_Q

catalog :

147340

citations: 1

Reference
Keskeny C, Raisch T, Sgromo A, Igreja C, Bhandari D, Weichenrieder O, et al. A conserved CAF40-binding motif in metazoan NOT4 mediates association with the CCR4-NOT complex. Genes Dev. 2019;33:236-252 pubmed publisher

product information

Catalog Number :

147340

Product Name :

pAc5.1B-lambdaN-HA-DmNot4-dsRNAres_Q

article :

doi	10.1101/gad.320952.118
id	28211244
pubmed_id	30692204

bacterial resistance :

Ampicillin

cloning :

backbone

Unknown

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact help@addgene.org before placing your order to request that our lab fully sequence the plasmid.

growth strain :

Insect Expression of DmNot4-dsRNAres

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CG5244, cg5251, CG5251, Dmel CG31716, not3/5, not4, NOT4
gene	CG31716
id	34416

genbank_ids

mutation

7 silent mutations and 6 non silent mutations G207S, S295P, S402G, A405T, V537D, I899M and a deletion of QQQ 775-777 compared to the sequence given by NCBI:NM_164919.2; the annotated variations were found in two different clones from 2 independent experiments.

name

DmNot4-dsRNAres

shRNA_sequence

size

species

7227

Drosophila melanogaster