This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCGN H-Ras 61L, 189L
catalog :
14721
citations: 1
Reference
Fiordalisi J, Johnson R, Ulkü A, Der C, Cox A. Mammalian expression vectors for Ras family proteins: generation and use of expression constructs to analyze Ras family function. Methods Enzymol. 2001;332:3-36 pubmed
product information
Catalog Number :
14721
Product Name :
pCGN H-Ras 61L, 189L
article :
doi
id1123
pubmed_id11305105
bacterial resistance :
Ampicillin
cloning :
backbonepCGN
backbone_mutation
backbone_origin
backbone_size7500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Prenylation mutant: modified by geranylgeranylation, still undergoies remaining CAAX-signaled modifications and palmitoylation; retains transforming activity.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
geneHRAS
id3265
genbank_ids
mutation61L, 189L
nameH-ras
shRNA_sequence
size580
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHA
plasmid copy :
Channing Der
resistance markers :
96
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA