This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABE H-Ras 61L, 186S
catalog :
14719
citations: 1
Reference
Fiordalisi J, Johnson R, Ulkü A, Der C, Cox A. Mammalian expression vectors for Ras family proteins: generation and use of expression constructs to analyze Ras family function. Methods Enzymol. 2001;332:3-36 pubmed
product information
Catalog Number :
14719
Product Name :
pBABE H-Ras 61L, 186S
article :
doi
id1123
pubmed_id11305105
bacterial resistance :
Ampicillin
cloning :
backbonepBABE puro
backbone_mutation
backbone_origin
backbone_size5169
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Prenylation mutant: does not undergo farnesylation or any other CAAX-signaled posttranslational modification, including palmitoylation; cytosolic, nontransforming; dominant inhibitory for activated Ras, by competition for effectors.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3pBABE 3'
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
geneHRAS
id3265
genbank_ids
mutation61L, 186S
nameH-ras
shRNA_sequence
size580
species
9606
Homo sapiens
tags
plasmid copy :
Channing Der
resistance markers :
96
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA