pRK5 BARK1 minigene | Addgene 14695 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRK5 BARK1 minigene

catalog :

14695

citations: 3

Reference
Diao X, Yao L, Ma J, Zhang T, Bai H, Suo Z, et al. Analgesic action of adenosine A1 receptor involves the dephosphorylation of glycine receptor α1ins subunit in spinal dorsal horn of mice. Neuropharmacology. 2020;176:108219 pubmed publisher
Alkhatib O, da Costa R, Gentry C, Quallo T, Bevan S, Andersson D. Promiscuous G-Protein-Coupled Receptor Inhibition of Transient Receptor Potential Melastatin 3 Ion Channels by Gβγ Subunits. J Neurosci. 2019;39:7840-7852 pubmed publisher
Koch W, Hawes B, Inglese J, Luttrell L, Lefkowitz R. Cellular expression of the carboxyl terminus of a G protein-coupled receptor kinase attenuates G beta gamma-mediated signaling. J Biol Chem. 1994;269:6193-7 pubmed

product information

Catalog Number :

14695

Product Name :

pRK5 BARK1 minigene

article :

doi
id	1333
pubmed_id	8119963

bacterial resistance :

Ampicillin

cloning :

backbone

pRK5

backbone_mutation

backbone_origin

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The cDNA encoding bovine BARK1 residues Gly495 to Leu689 was amplified using PCR to construct a minigene casette with newly engineered 5' and 3' ends (see Author's map). The 5' end contained an EcoRI site for subcloning, followed by the kozak sequence 5'-GCCGCCACCATGG-3'. A bclI site was inserted at the 3' end immediately following the stop codon. The final BARK1 minigene construct contains the 3'UTR of the human beta-globin gene and a polyadenylation signal.

origin :

pi :

alt_names

beta ARK1

beta adrenergic receptor kinase

ADRBK1

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	EcoRI
promoter
sequencing_primer_3
sequencing_primer_5	Sp6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ADRBK1
gene	GRK2
id	282682

genbank_ids

mutation

BARK1 amino acids 495-689

name

BARK1 minigene

shRNA_sequence

size

species

9913

Bos taurus