This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRK5 bovine GRK5
catalog :
14690
citations: 1
| Reference |
|---|
Pitcher J, Fredericks Z, Stone W, Premont R, Stoffel R, Koch W, et al. Phosphatidylinositol 4,5-bisphosphate (PIP2)-enhanced G protein-coupled receptor kinase (GRK) activity. Location, structure, and regulation of the PIP2 binding site distinguishes the GRK subfamilies. J Biol Chem. 1996;271:24907-13 pubmed
|
product information
Catalog Number :
14690
Product Name :
pRK5 bovine GRK5
article :
| doi | 10.1074/jbc.271.40.24907 |
| id | 1330 |
| pubmed_id | 8798768 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pRK5 | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | 4700 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
The cDNA encoding bovine GRK5 was modified to remove all 3' and 5' untranslated regions. Using standard polymerase chain reaction techniques, a new 5' end containing EcoRI, BglII, and Kozak consensus sequences was added before the start codon, and a new 3' end containing an XbaI site was inserted immediately after the translational stop codon. The amplified fragment was inserted into the EcoRI/XbaI sites of pRK5.
origin :
37
pi :
|
resistance markers :
| 285 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments