pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J | Addgene 146702 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J

catalog :

146702

citations: 1

Reference
Chen Y, Boland A, Kuzuoğlu Öztürk D, Bawankar P, Loh B, Chang C, et al. A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing. Mol Cell. 2014;54:737-50 pubmed publisher

product information

Catalog Number :

146702

Product Name :

pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J

article :

doi	10.1016/j.molcel.2014.03.034
id	28211235
pubmed_id	24768540

bacterial resistance :

Ampicillin

cloning :

backbone

Unknown

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact help@addgene.org before placing your order to request that our lab fully sequence the plasmid.

growth strain :

Insect Expression of DmNot1_1710-2505

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG34407, CG1874, CG1884, CG34407, Dmel CG34407, Dmel_CG1874, Dmel_CG1884, NOT, NOT1, anon-EST:Liang-1.29, anon-WO0118547.221, clone 1.29, not1
gene	Not1
id	5740252

genbank_ids

mutation

two non silent mutations (A1747T, G1999S) and one silent mutation compared to the sequence given by NCBI: NM_001103772.2

name

DmNot1_1710-2505

shRNA_sequence

size

species

7227

Drosophila melanogaster