pMT-NTAP-DmNot3_J | Addgene 146651 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMT-NTAP-DmNot3_J

catalog :

146651

product information

Catalog Number :

146651

Product Name :

pMT-NTAP-DmNot3_J

article :

doi
id	2602
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pMK33-NTAP

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

growth notes :

pMK33-NTAP and pMK33-CTAP vectors are designed for making proteinfusions tagged with the TAP tag and for generating stableDrosophila cell lines under Hygromycin selection. TAP fusions areinducible with copper sulfate. The parental vector, pMK33 (pMt/Hy),was created by Michael Koelle. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact help@addgene.org before placing your order to request that our lab fully sequence the plasmid.

growth strain :

Insect Expression of DmNot3

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG8426, CG8426, CNOT3, Dmel CG8426, NOT3, NOT3.5, NOT3/5, l(2)NC136, not3, not3/5
gene	Not3
id	35501

genbank_ids

mutation

One silent and three non silent mutations E90G, K110T and P602S compared to the sequence given by NCBI: NM_136332.2

name

DmNot3

shRNA_sequence

size

species

7227

Drosophila melanogaster