Rev CDK9 (D167N) (P#858) | Addgene 14635 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Rev CDK9 (D167N) (P#858)

catalog :

14635

citations: 1

Reference
Fujinaga K, Cujec T, Peng J, Garriga J, Price D, Grana X, et al. The ability of positive transcription elongation factor B to transactivate human immunodeficiency virus transcription depends on a functional kinase domain, cyclin T1, and Tat. J Virol. 1998;72:7154-9 pubmed

product information

Catalog Number :

14635

Product Name :

Rev CDK9 (D167N) (P#858)

article :

doi
id	1308
pubmed_id	9696809

bacterial resistance :

Ampicillin

cloning :

backbone

pSPORT-Rev

backbone_mutation

backbone_origin

backbone_size

4200

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

3' (lowercase letters indicate the PvuII site) and 5' CAGACGGAGTTTGAGCGCGTCTTCCTCGAGAGGGGCCGGCG 3'. Amplified products (1.1 kbp) were restricted with PvuII and subcloned into pSPORTRev (PvuII).
GCGATCcagctgGAGGCGGCCATGGCAAAGCAGTACGAC
TCGGT
To construct pSPORTRev, Rev coding sequences (350 bp) were amplified by PCR from pcRev, restricted with AflII and SacI, and cloned into the PstI and SacI sites of pSV SPORT 1 (Gibco BRL, Gaithersburg, Md.), which contains modified polylinker sequences . CDK9 (D167N) was amplified by PCR from pRc/CMVCDK9D-NHA [pCDK9(D167N) with the oligonucleotides 5'

origin :

pi :

alt_names

cyclin dependent kinase 9

cdk9

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	SalI
promoter
sequencing_primer_3	T7
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C-2k, CDC2L4, CTK1, PITALRE, TAK
gene	CDK9
id	1025

genbank_ids

mutation

D167N. Kinase negative mutant.

name

cdk9 D167N

shRNA_sequence

size

1100

species

9606

Homo sapiens