This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV Lex VP16 (F442A) HA (P#1710)
catalog :
14595
citations: 1
Reference
Kurosu T, Peterlin B. VP16 and ubiquitin; binding of P-TEFb via its activation domain and ubiquitin facilitates elongation of transcription of target genes. Curr Biol. 2004;14:1112-6 pubmed
product information
Catalog Number :
14595
Product Name :
pCMV Lex VP16 (F442A) HA (P#1710)
article :
doi10.1016/j.cub.2004.06.020
id1299
pubmed_id15203006
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA
backbone_mutation
backbone_originInvitrogen
backbone_size5600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
Lex
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationThe VP16 sequence contains an F442A mutation. The substitution of alanine for phenylanine at position 442 has previously been shown to affect the transcriptional activity of VP16.
nameLexA
shRNA_sequence
size837
species
E. coli
tags
locationC terminal on insert
tagHA
locationC terminal on insert
tagVP16 (F442A)
resistance markers :
390
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA