This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)
catalog :
145843
citations: 6
Reference
Wang Z, Pan Q, Ma L, Zhao J, McIntosh F, Liu Z, et al. Anthracyclines inhibit SARS-CoV-2 infection. Virus Res. 2023;334:199164 pubmed publisher
Kim T, Kim J, Kwon H, Jeon S, Lee S, Jung H, et al. Astersaponin I from Aster koraiensis is a natural viral fusion blocker that inhibits the infection of SARS-CoV-2 variants and syncytium formation. Antiviral Res. 2022;208:105428 pubmed publisher
Durdagi S, Avsar T, Orhan M, Serhatlı M, Balcioglu B, Ozturk H, et al. The neutralization effect of montelukaston SARS-CoV-2 is shown by multiscale in silicosimulations and combined in vitro studies. Mol Ther. 2021;: pubmed publisher
Dogan K, Erol E, Didem Orhan M, Degirmenci Z, Kan T, Gungor A, et al. Instant determination of the artemisinin from various Artemisia annua L. extracts by LC-ESI-MS/MS and their in-silico modelling and in vitro antiviral activity studies against SARS-CoV-2. Phytochem Anal. 2021;: pubmed publisher
Kim T, Jeon S, Jang Y, Gotina L, Won J, Ju Y, et al. Platycodin D, a natural component of Platycodon grandiflorum, prevents both lysosome- and TMPRSS2-driven SARS-CoV-2 infection by hindering membrane fusion. Exp Mol Med. 2021;53:956-972 pubmed publisher
Rebendenne A, Valadão A, Tauziet M, Maarifi G, Bonaventure B, McKellar J, et al. SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells. J Virol. 2021;: pubmed publisher
product information
Catalog Number :
145843
Product Name :
RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)
article :
doi10.1128/JVI.02415-20
id28211215
pubmed_id33514628
bacterial resistance :
Ampicillin
cloning :
backboneRRL.sin.cPPT.SFFV/IRES-neo.WPRE.
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
NM_001135099 with 1 mutation G8V
growth strain :
Lentiviral vector genome to ectopically express human TMPRSS2 variant 1
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NotI
promoterSFFV
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPRSS10
geneTMPRSS2
id7113
genbank_ids
mutationG8V
namehuman TMPRSS2 variant 1
shRNA_sequence
size1590
species
9606
Homo sapiens
tags
plasmid copy :
lentiviral puroR: puroR replaced by neoR RRL.sin.cPPT.SFFV/IRES-neo.WPRE
resistance markers :
4850
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA