This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136)
catalog :
145842
citations: 4
Reference
Bonaventure B, Rebendenne A, Chaves Valad xe3 o A, Arnaud Arnould M, Gracias S, García de Gracia F, et al. The DEAD box RNA helicase DDX42 is an intrinsic inhibitor of positive-strand RNA viruses. EMBO Rep. 2022;23:e54061 pubmed publisher
Rebendenne A, Roy P, Bonaventure B, Chaves Valad xe3 o A, Desmarets L, Arnaud Arnould M, et al. Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs. Nat Genet. 2022;54:1090-1102 pubmed publisher
Goujon C, Rebendenne A, Roy P, Bonaventure B, Valadao A, Desmarets L, et al. Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs. Res Sq. 2021;: pubmed publisher
Rebendenne A, Valadão A, Tauziet M, Maarifi G, Bonaventure B, McKellar J, et al. SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells. J Virol. 2021;: pubmed publisher
product information
Catalog Number :
145842
Product Name :
RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136)
article :
doi10.1128/JVI.02415-20
id28211215
pubmed_id33514628
bacterial resistance :
Ampicillin
cloning :
backboneRRL.sin.cPPT.SFFV.WPRE.
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentiviral vector genome to ectopically express human Ace2
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5XhoI
promoterSFFV
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACEH
geneACE2
id59272
genbank_ids
mutation
namehuman Ace2
shRNA_sequence
size2418
species
9606
Homo sapiens
tags
plasmid copy :
RRL.sin.cPPT.SFFV.WPRE
resistance markers :
4850
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA