This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RRL.sin.cPPT.SFFV/Ace2.IRES-neo.WPRE (MT129)
catalog :
145840
citations: 2
Reference
Ugalde A, Bretones G, Rodr xed guez D, Quesada V, Llorente F, Fern xe1 ndez Delgado R, et al. Autophagy-linked plasma and lysosomal membrane protein PLAC8 is a key host factor for SARS-CoV-2 entry into human cells. EMBO J. 2022;41:e110727 pubmed publisher
Rebendenne A, Valadão A, Tauziet M, Maarifi G, Bonaventure B, McKellar J, et al. SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells. J Virol. 2021;: pubmed publisher
product information
Catalog Number :
145840
Product Name :
RRL.sin.cPPT.SFFV/Ace2.IRES-neo.WPRE (MT129)
article :
doi10.1128/JVI.02415-20
id28211215
pubmed_id33514628
bacterial resistance :
Ampicillin
cloning :
backboneRRL.sin.cPPT.SFFV/IRES-neo.WPRE.
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentiviral vector genome to ectopically express human Ace2
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NotI
promoterSFFV
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACEH
geneACE2
id59272
genbank_ids
mutation
namehuman Ace2
shRNA_sequence
size2418
species
9606
Homo sapiens
tags
plasmid copy :
lentiviral puroR: puroR replaced by neoR RRL.sin.cPPT.SFFV/IRES-neo.WPRE
resistance markers :
4850
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA