This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCIS-rPALcc
catalog :
145804
citations: 1
Reference
Kolhekar A, Bell J, Shiozaki E, Jin L, Keutmann H, Hand T, et al. Essential features of the catalytic core of peptidyl-alpha-hydroxyglycine alpha-amidating lyase. Biochemistry. 2002;41:12384-94 pubmed
product information
Catalog Number :
145804
Product Name :
pCIS-rPALcc
article :
doi10.1021/bi0260280
id28211210
pubmed_id12369828
bacterial resistance :
Ampicillin
cloning :
backbonepCIS
backbone_mutation
backbone_originCornelia Gorman
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Xba1
cloning_site_5Hind3
promoterCMV
sequencing_primer_3TGGTGTTGATCTTACGTCAC
sequencing_primer_5CTTGAGGTGTGGCAGGCTTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPHM
genePam
id25508
genbank_ids
mutationchanged serine 767 to alanine to prevent glycosylation
namerat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase)
shRNA_sequence
size1350
species
10116
Rattus norvegicus
tags
resistance markers :
750
751
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA