JP1520 PIK3CA E545K HA | Addgene 14571 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

JP1520 PIK3CA E545K HA

catalog :

14571

citations: 3

Reference
Elloul S, Kedrin D, Knoblauch N, Beck A, Toker A. The adherens junction protein afadin is an AKT substrate that regulates breast cancer cell migration. Mol Cancer Res. 2014;12:464-76 pubmed publisher
Paget J, Restall I, Daneshmand M, Mersereau J, Simard M, Parolin D, et al. Repression of cancer cell senescence by PKC?. Oncogene. 2012;31:3584-96 pubmed publisher
Isakoff S, Engelman J, Irie H, Luo J, Brachmann S, Pearline R, et al. Breast cancer-associated PIK3CA mutations are oncogenic in mammary epithelial cells. Cancer Res. 2005;65:10992-1000 pubmed

product information

Catalog Number :

14571

Product Name :

JP1520 PIK3CA E545K HA

article :

doi	10.1158/0008-5472.CAN-05-2612
id	1286
pubmed_id	16322248

bacterial resistance :

Ampicillin

cloning :

backbone

JP1520

backbone_mutation

backbone_origin

Jeff Engelman

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

Hemagglutinin (HA) tagged bovine PIK3CA cDNA was subcloned into the pDNR-Dual vector (Clontech, Mountain View, CA), and site-directed mutagenesis (Quick Change, Stratagene, La Jolla, CA) was used to generate the E545K mutation. The cDNA was shuttled into the JP1520 retroviral vector using the BD Creator System (Clontech).

origin :

pi :

alt_names

PIK3CA

p110 alpha

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	PIK3CA
id	282306

genbank_ids

mutation

E545K

name

PIK3CA E545K

shRNA_sequence

size

species

9913

Bos taurus