This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
V249 pCEP-4HA B56delta
catalog :
14536
citations: 5
Reference
Li J, Zhou J, Mu X, Shen S, Xu X, Luo Y, et al. Regulation of XPO5 phosphorylation by PP2A in hepatocellular carcinoma. MedComm (2020). 2022;3:e125 pubmed publisher
Wu D, De Wever V, Derua R, Winkler C, Beullens M, Van Eynde A, et al. A substrate-trapping strategy for protein phosphatase PP1 holoenzymes using hypoactive subunit fusions. J Biol Chem. 2018;293:15152-15162 pubmed publisher
Suryavanshi S, Jadhav S, Anderson K, Katsonis P, Lichtarge O, McConnell B. Human muscle-specific A-kinase anchoring protein polymorphisms modulate the susceptibility to cardiovascular diseases by altering cAMP/PKA signaling. Am J Physiol Heart Circ Physiol. 2018;315:H109-H121 pubmed publisher
Greenwood E, Matheson N, Wals K, van den Boomen D, Antrobus R, Williamson J, et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. elife. 2016;5: pubmed publisher
Seeling J, Miller J, Gil R, Moon R, White R, Virshup D. Regulation of beta-catenin signaling by the B56 subunit of protein phosphatase 2A. Science. 1999;283:2089-91 pubmed
product information
Catalog Number :
14536
Product Name :
V249 pCEP-4HA B56delta
article :
doi10.1126/science.283.5410.2089
id1281
pubmed_id10092233
bacterial resistance :
Ampicillin
cloning :
backbonepCEP4 (modified)
backbone_mutation
backbone_originInvitrogen
backbone_size10186
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene. Please note that the Addgene verified sequence finds an R210H mutation in PPP2R5D. Other differences compared to the depositor sequence do not affect plasmid function.
origin :
37
pi :
alt_names
B' delta
PR61 delta, B56D
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5Not1
promoter
sequencing_primer_3EBV Reverse (invitrogen)
sequencing_primer_5pCEP forward (invitrogen)
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesB56D, B56delta, HJS1, MRD35
genePPP2R5D
id5528
genbank_ids
L76702
mutationR210H
namePPP2R5D B56 delta regulatory subunit of PP2A
shRNA_sequence
size2100
species
9606
Homo sapiens
tags
locationN terminal on insert
tag4 HA
resistance markers :
552
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA