pcDNA3-sACE2(WT)-Fc(IgG1) | Addgene 145163 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3-sACE2(WT)-Fc(IgG1)

catalog :

145163

citations: 9

Reference
Stewart H, Palmulli R, Johansen K, McGovern N, Shehata O, Carnell G, et al. Tetherin antagonism by SARS-CoV-2 ORF3a and spike protein enhances virus release. EMBO Rep. 2023;24:e57224 pubmed publisher
Kumar C, Singh B, Rizvi Z, Parray H, Verma J, Ghosh S, et al. Virus-Like Particles of SARS-CoV-2 as Virus Surrogates: Morphology, Immunogenicity, and Internalization in Neuronal Cells. ACS Infect Dis. 2022;8:2119-2132 pubmed publisher
Dickey T, Tang W, Butler B, Ouahes T, Orr Gonzalez S, Salinas N, et al. Design of the SARS-CoV-2 RBD vaccine antigen improves neutralizing antibody response. Sci Adv. 2022;8:eabq8276 pubmed publisher
Capraz T, Kienzl N, Laurent E, Perthold J, Föderl Höbenreich E, Grünwald Gruber C, et al. Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptor. elife. 2021;10: pubmed publisher
Shrivastava T, Singh B, Rizvi Z, Verma R, Goswami S, Vishwakarma P, et al. Comparative Immunomodulatory Evaluation of the Receptor Binding Domain of the SARS-CoV-2 Spike Protein; a Potential Vaccine Candidate Which Imparts Potent Humoral and Th1 Type Immune Response in a Mouse Model. Front Immunol. 2021;12:641447 pubmed publisher
Dickey T, Tang W, Butler B, Ouahes T, Orr Gonzalez S, Salinas N, et al. Design of the SARS-CoV-2 RBD vaccine antigen improves neutralizing antibody response. bioRxiv. 2021;: pubmed publisher
Bosnjak B, Stein S, Willenzon S, Cordes A, Puppe W, Bernhardt G, et al. Low serum neutralizing anti-SARS-CoV-2 S antibody levels in mildly affected COVID-19 convalescent patients revealed by two different detection methods. Cell Mol Immunol. 2020;: pubmed publisher
Chan K, Dorosky D, Sharma P, Abbasi S, Dye J, Kranz D, et al. Engineering human ACE2 to optimize binding to the spike protein of SARS coronavirus 2. Science. 2020;369:1261-1265 pubmed publisher
Procko E. The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2. bioRxiv. 2020;: pubmed publisher

product information

Catalog Number :

145163

Product Name :

pcDNA3-sACE2(WT)-Fc(IgG1)

article :

doi	10.1126/science.abc0870
id	28211097
pubmed_id	32753553

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1(+)

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5332

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.

growth strain :

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)

origin :

pi :

alt_names

ACE2

peptidyl-dipeptidase A

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NheI
promoter	CMV
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ACEH
gene	ACE2
id	59272

genbank_ids

NM_021804.1

mutation

Extracellular, soluble protease domain (a.a. M1-D615)

name

Angiotensin-converting enzyme 2

shRNA_sequence

size

2556

species

9606

Homo sapiens