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product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1-hACE2
catalog :
145033
citations: 22
Reference
Engler M, Albers D, von Maltitz P, Gro xdf R, M xfc nch J, Cirstea I. ACE2-EGFR-MAPK signaling contributes to SARS-CoV-2 infection. Life Sci Alliance. 2023;6: pubmed publisher
Mart xed nez M xe1 rmol R, Giordano Santini R, Kaulich E, Cho A, Przybyla M, Riyadh M, et al. SARS-CoV-2 infection and viral fusogens cause neuronal and glial fusion that compromises neuronal activity. Sci Adv. 2023;9:eadg2248 pubmed publisher
Baciu D, Dinu S, Palade B, Onu A, Costache A. Comparison of truncated human angiotensin-converting enzyme 2 (hACE2) expression in pET28a(+) versus pET-SUMO vector and two Escherichia coli strains. Adv Med Sci. 2023;68:61-70 pubmed publisher
Andreano E, Paciello I, Pierleoni G, Piccini G, Abbiento V, Antonelli G, et al. B cell analyses after SARS-CoV-2 mRNA third vaccination reveals a hybrid immunity like antibody response. Nat Commun. 2023;14:53 pubmed publisher
Charles J, McCann N, Ploplis V, Castellino F. Spike protein receptor-binding domains from SARS-CoV-2 variants of interest bind human ACE2 more tightly than the prototype spike protein. Biochem Biophys Res Commun. 2023;641:61-66 pubmed publisher
Brasu N, Elia I, Russo V, Montacchiesi G, Stabile S, De Intinis C, et al. Memory CD8+ T cell diversity and B cell responses correlate with protection against SARS-CoV-2 following mRNA vaccination. Nat Immunol. 2022;23:1445-1456 pubmed publisher
Compagnone M, Pinto E, Salvatori E, Lione L, Conforti A, Marchese S, et al. DNA-Vaccine-Induced Immune Response Correlates with Lower Viral SARS-CoV-2 Titers in a Ferret Model. Vaccines (Basel). 2022;10: pubmed publisher
Aicher S, Streicher F, Chazal M, Planas D, Luo D, Buchrieser J, et al. Species-Specific Molecular Barriers to SARS-CoV-2 Replication in Bat Cells. J Virol. 2022;96:e0060822 pubmed publisher
Andreano E, Paciello I, Marchese S, Donnici L, Pierleoni G, Piccini G, et al. Anatomy of Omicron BA.1 and BA.2 neutralizing antibodies in COVID-19 mRNA vaccinees. Nat Commun. 2022;13:3375 pubmed publisher
Vanhulle E, Stroobants J, Provinciael B, Camps A, Noppen S, Maes P, et al. SARS-CoV-2 Permissive glioblastoma cell line for high throughput antiviral screening. Antiviral Res. 2022;203:105342 pubmed publisher
Torres J, Ozorowski G, Andreano E, Liu H, Copps J, Piccini G, et al. Structural insights of a highly potent pan-neutralizing SARS-CoV-2 human monoclonal antibody. Proc Natl Acad Sci U S A. 2022;119:e2120976119 pubmed publisher
Shah M, Ung Moon S, Hyun Kim J, Thanh Thao T, Goo Woo H. SARS-CoV-2 pan-variant inhibitory peptides deter S1-ACE2 interaction and neutralize delta and omicron pseudoviruses. Comput Struct Biotechnol J. 2022;20:2042-2056 pubmed publisher
Francesconi O, Donnici L, Fragai M, Pesce E, Bombaci M, Fasciani A, et al. Synthetic carbohydrate-binding agents neutralize SARS-CoV-2 by inhibiting binding of the spike protein to ACE2. iScience. 2022;25:104239 pubmed publisher
Barrow K, Wang Y, Yu R, Zhu J, Yang G. H2S protects from oxidative stress-driven ACE2 expression and cardiac aging. Mol Cell Biochem. 2022;477:1393-1403 pubmed publisher
Villa A, Brunialti E, Dellavedova J, Meda C, Rebecchi M, Conti M, et al. DNA aptamers masking angiotensin converting enzyme 2 as an innovative way to treat SARS-CoV-2 pandemic. Pharmacol Res. 2022;175:105982 pubmed publisher
Shin J, Toyoda S, Nishitani S, Fukuhara A, Kita S, Otsuki M, et al. Possible Involvement of Adipose Tissue in Patients With Older Age, Obesity, and Diabetes With SARS-CoV-2 Infection (COVID-19) via GRP78 (BIP/HSPA5): Significance of Hyperinsulinemia Management in COVID-19. Diabetes. 2021;70:2745-2755 pubmed publisher
Brune K, Lieknina I, Sutov G, Morris A, Jovicevic D, Kalnins G, et al. N-Terminal Modification of Gly-His-Tagged Proteins with Azidogluconolactone. Chembiochem. 2021;22:3199-3207 pubmed publisher
Youn J, Zhang Y, Wu Y, Cannesson M, Cai H. Therapeutic application of estrogen for COVID-19: Attenuation of SARS-CoV-2 spike protein and IL-6 stimulated, ACE2-dependent NOX2 activation, ROS production and MCP-1 upregulation in endothelial cells. Redox Biol. 2021;46:102099 pubmed publisher
Li X, Lidsky P, Xiao Y, Wu C, Garcia Knight M, Yang J, et al. Ethacridine inhibits SARS-CoV-2 by inactivating viral particles. PLoS Pathog. 2021;17:e1009898 pubmed publisher
García Ayllón M, Moreno Pérez Ó, García Arriaza J, Ramos Rincón J, Cortés Gómez M, Brinkmalm G, et al. Plasma ACE2 species are differentially altered in COVID-19 patients. FASEB J. 2021;35:e21745 pubmed publisher
Li X, Lidsky P, Xiao Y, Wu C, Garcia Knight M, Yang J, et al. Ethacridine inhibits SARS-CoV-2 by inactivating viral particles in cellular models. bioRxiv. 2020;: pubmed publisher
Shang J, Ye G, Shi K, Wan Y, Luo C, Aihara H, et al. Structural basis of receptor recognition by SARS-CoV-2. Nature. 2020;581:221-224 pubmed publisher
product information
Catalog Number :
145033
Product Name :
pcDNA3.1-hACE2
article :
doi10.1038/s41586-020-2179-y
id28211168
pubmed_id32225175
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_origin
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Express human ACE2 with C9 tag at C-terminal in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3none
cloning_site_5XbaI
promoterCMV
sequencing_primer_3BGH-R
sequencing_primer_5T7-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACEH
geneACE2
id59272
genbank_ids
mutation
namehuman ACE2
shRNA_sequence
size2445
species
9606
Homo sapiens
tags
locationC terminal on insert
tagC9
resistance markers :
4918
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA