This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p3259 pCMV hUBC9 mt HA
catalog :
14439
citations: 4
Reference
Rajput M, Abdelsalam K, Darweesh M, Braun L, Kerkvliet J, Hoppe A, et al. Both cytopathic and non-cytopathic bovine viral diarrhea virus (BVDV) induced autophagy at a similar rate. Vet Immunol Immunopathol. 2017;193-194:1-9 pubmed publisher
Pagano C, di Martino O, Ruggiero G, Maria Guarino A, Mueller N, Siauciunaite R, et al. The tumor-associated YB-1 protein: new player in the circadian control of cell proliferation. Oncotarget. 2017;8:6193-6205 pubmed publisher
Foran E, Rosenblum L, Bogush A, Pasinelli P, Trotti D. Sumoylation of the astroglial glutamate transporter EAAT2 governs its intracellular compartmentalization. Glia. 2014;62:1241-53 pubmed publisher
Yasugi T, Howley P. Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9. Nucleic Acids Res. 1996;24:2005-10 pubmed
product information
Catalog Number :
14439
Product Name :
p3259 pCMV hUBC9 mt HA
article :
doi10.1093/nar/24.11.2005
id1266
pubmed_id8668529
bacterial resistance :
Ampicillin
cloning :
backbonepCMV4
backbone_mutation
backbone_origin
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
A fragment containing the full coding region of hubc9 C93S cDNA along with the influenza virus hemagglutinin 1 epitope (HA) sequence (YPYDVPDYA) at the 3 -end was produced with appropriate synthetic oligonucleotides using PCR. The fragment was then cloned into the pCMV4 vector at the BglII site.
origin :
37
pi :
alt_names
UBC 9
hUBC9
cloning
clone_methodRestriction Enzyme
cloning_site_3BclI/BglII
cloning_site_5BglII
promoterCMV
sequencing_primer_3hGH-pA-R (5'-CCAGCTTGGTTCCCAATAGA-3')
sequencing_primer_5CMV-F
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesC358B7.1, P18, UBC9
geneUBE2I
id7329
genbank_ids
mutationCys93Ser mutation (TGC changed to AGC)
nameUBC9 C93S
shRNA_sequence
size500
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHA
resistance markers :
209
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA