p3258 pCMV hUBC9 wt HA | Addgene 14438 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

p3258 pCMV hUBC9 wt HA

catalog :

14438

citations: 8

Reference
Welch M, Jansen L, Baro D. SUMOylation of the Kv4.2 Ternary Complex Increases Surface Expression and Current Amplitude by Reducing Internalization in HEK 293 Cells. Front Mol Neurosci. 2021;14:757278 pubmed publisher
Rauth S, Karmakar S, Shah A, Seshacharyulu P, Nimmakayala R, Ganguly K, et al. SUMO Modification of PAF1/PD2 Enables PML Interaction and Promotes Radiation Resistance in Pancreatic Ductal Adenocarcinoma. Mol Cell Biol. 2021;41:e0013521 pubmed publisher
Kaci A, Keindl M, Solheim M, Njølstad P, Bjørkhaug L, Aukrust I. The E3 SUMO ligase PIAS? is a novel interaction partner regulating the activity of diabetes associated hepatocyte nuclear factor-1?. Sci Rep. 2018;8:12780 pubmed publisher
Johansson B, Fjeld K, Solheim M, Shirakawa J, Zhang E, Keindl M, et al. Nuclear import of glucokinase in pancreatic beta-cells is mediated by a nuclear localization signal and modulated by SUMOylation. Mol Cell Endocrinol. 2017;454:146-157 pubmed publisher
Parker A, Welch M, Forster L, Tasneem S, Dubhashi J, Baro D. SUMOylation of the Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 2 Increases Surface Expression and the Maximal Conductance of the Hyperpolarization-Activated Current. Front Mol Neurosci. 2016;9:168 pubmed publisher
Liu Y, Zheng Z, Shu B, Meng J, Zhang Y, Zheng C, et al. SUMO Modification Stabilizes Enterovirus 71 Polymerase 3D To Facilitate Viral Replication. J Virol. 2016;90:10472-10485 pubmed publisher
Choi J, Park J, Park S, Lee H, Han S, Park K, et al. Regulation of mGluR7 trafficking by SUMOylation in neurons. Neuropharmacology. 2016;102:229-35 pubmed publisher
Yasugi T, Howley P. Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9. Nucleic Acids Res. 1996;24:2005-10 pubmed

product information

Catalog Number :

14438

Product Name :

p3258 pCMV hUBC9 wt HA

article :

doi	10.1093/nar/24.11.2005
id	1266
pubmed_id	8668529

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV4

backbone_mutation

backbone_origin

backbone_size

4900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

A fragment containing the full coding region of hubc9 cDNA along with the influenza virus hemagglutinin 1 epitope (HA) sequence (YPYDVPDYA) at the 3 -end was produced with appropriate synthetic oligonucleotides using PCR. The fragment was then cloned into the pCMV4 vector at the BglII site.

origin :

pi :

alt_names

UBC 9

hUBC9

cloning

clone_method	Restriction Enzyme
cloning_site_3	BclI/BglII
cloning_site_5	BglII
promoter
sequencing_primer_3
sequencing_primer_5	CMV forward
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	C358B7.1, P18, UBC9
gene	UBE2I
id	7329

genbank_ids

mutation

name

UBC9

shRNA_sequence

size

500

species

9606

Homo sapiens