This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mRFP-Rab5
catalog :
14437
citations: 56
Reference
Bittel D, Jaiswal J. Early Endosomes Undergo Calcium-Triggered Exocytosis and Enable Repair of Diffuse and Focal Plasma Membrane Injury. Adv Sci (Weinh). 2023;10:e2300245 pubmed publisher
York H, Joshi K, Wright C, Kreplin L, Rodgers S, Moorthi U, et al. Deterministic early endosomal maturations emerge from a stochastic trigger-and-convert mechanism. Nat Commun. 2023;14:4652 pubmed publisher
Kumar N, Taily I, Singh C, Kumar S, Rajmani R, Chakraborty D, et al. Identification of diphenylurea derivatives as novel endocytosis inhibitors that demonstrate broad-spectrum activity against SARS-CoV-2 and influenza A virus both in vitro and in vivo. PLoS Pathog. 2023;19:e1011358 pubmed publisher
Bai Y, Yu G, Zhou H, Amarasinghe O, Zhou Y, Zhu P, et al. PTP4A2 promotes lysophagy by dephosphorylation of VCP/p97 at Tyr805. Autophagy. 2022;:1-20 pubmed publisher
Yuan C, Suo X, Duan Y, Li X, Shi L, Cao L, et al. Comprehensive Subcellular Localization of Swine Acute Diarrhea Syndrome Coronavirus Proteins. J Virol. 2022;96:e0077222 pubmed publisher
Taniguchi S, Ito Y, Kiritani H, Maruo A, Sakai R, Ono Y, et al. The Ubiquitin Ligase RNF34 Participates in the Peripheral Quality Control of CFTR (RNF34 Role in CFTR PeriQC). Front Mol Biosci. 2022;9:840649 pubmed publisher
Nørgård M, Steffensen L, Hansen D, Füchtbauer E, Engelund M, Dimke H, et al. A new transgene mouse model using an extravesicular EGFP tag enables affinity isolation of cell-specific extracellular vesicles. Sci Rep. 2022;12:496 pubmed publisher
Trofimenko E, Homma Y, Fukuda M, Widmann C. The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7. Cell Rep. 2021;37:109945 pubmed publisher
Wiatr K, Marczak Ł, Perot J, Brouillet E, Flament J, Figiel M. Broad Influence of Mutant Ataxin-3 on the Proteome of the Adult Brain, Young Neurons, and Axons Reveals Central Molecular Processes and Biomarkers in SCA3/MJD Using Knock-In Mouse Model. Front Mol Neurosci. 2021;14:658339 pubmed publisher
Sønder S, Häger S, Heitmann A, Frankel L, Dias C, Simonsen A, et al. Restructuring of the plasma membrane upon damage by LC3-associated macropinocytosis. Sci Adv. 2021;7: pubmed publisher
Jeckel P, Kriebel M, Volkmer H. Autism Spectrum Disorder Risk Factor Met Regulates the Organization of Inhibitory Synapses. Front Mol Neurosci. 2021;14:659856 pubmed publisher
Hao M, Yeo S, Turner K, Harold A, Yang Y, Zhang X, et al. Autophagy Blockade Limits HER2+ Breast Cancer Tumorigenesis by Perturbing HER2 Trafficking and Promoting Release Via Small Extracellular Vesicles. Dev Cell. 2021;56:341-355.e5 pubmed publisher
Arnold J, Schattschneider J, Blechner C, Krisp C, Schluter H, Schweizer M, et al. Tubulin Tyrosine Ligase Like 4 (TTLL4) overexpression in breast cancer cells is associated with brain metastasis and alters exosome biogenesis. J Exp Clin Cancer Res. 2020;39:205 pubmed publisher
Caudano F, Montalto G, Passalacqua M, Pronzato M, Fedele E, Ricciarelli R. cGMP favors the interaction between APP and BACE1 by inhibiting Rab5 GTPase activity. Sci Rep. 2020;10:1358 pubmed publisher
Kim M, Zhong Y, Jung K, Chai Y, Binas B. Basal-type lumenogenesis in extraembryonic endoderm stem cells models the early visceral endoderm. J Cell Sci. 2019;132: pubmed publisher
Edfawy M, Guedes J, Pereira M, Laranjo M, Carvalho M, Gao X, et al. Abnormal mGluR-mediated synaptic plasticity and autism-like behaviours in Gprasp2 mutant mice. Nat Commun. 2019;10:1431 pubmed publisher
Sakai R, Fukuda R, Unida S, Aki M, Ono Y, Endo A, et al. The integral function of the endocytic recycling compartment is regulated by RFFL-mediated ubiquitylation of Rab11 effectors. J Cell Sci. 2019;132: pubmed publisher
Li S, Ahmad I, Shi J, Wang B, Yu C, Zhang L, et al. Murine Leukemia Virus Glycosylated Gag Reduces Murine SERINC5 Protein Expression at Steady-State Levels via the Endosome/Lysosome Pathway to Counteract SERINC5 Antiretroviral Activity. J Virol. 2019;93: pubmed publisher
Heisler F, Pechmann Y, Wieser I, Altmeppen H, Veenendaal L, Muhia M, et al. Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression. Neuron. 2018;99:1155-1169.e9 pubmed publisher
Williams E, Glauser L, Tsika E, Jiang H, Islam S, Moore D. Parkin mediates the ubiquitination of VPS35 and modulates retromer-dependent endosomal sorting. Hum Mol Genet. 2018;27:3189-3205 pubmed publisher
Otero M, Fernandez Bessone I, Hallberg A, Cromberg L, De Rossi M, Saez T, et al. Proteasome stress leads to APP axonal transport defects by promoting its amyloidogenic processing in lysosomes. J Cell Sci. 2018;131: pubmed publisher
Shi J, Xiong R, Zhou T, Su P, Zhang X, Qiu X, et al. HIV-1 Nef Antagonizes SERINC5 Restriction by Downregulation of SERINC5 via the Endosome/Lysosome System. J Virol. 2018;92: pubmed publisher
Okiyoneda T, Veit G, Sakai R, Aki M, Fujihara T, Higashi M, et al. Chaperone-Independent Peripheral Quality Control of CFTR by RFFL E3 Ligase. Dev Cell. 2018;44:694-708.e7 pubmed publisher
Hsu T, Mo S, Hsu P, Lai M. c-FLIP is a target of the E3 ligase deltex1 in gastric cancer. Cell Death Dis. 2018;9:135 pubmed publisher
Niebert S, van Belle G, Vogelgesang S, Manzke T, Niebert M. The Serotonin Receptor Subtype 5b Specifically Interacts with Serotonin Receptor Subtype 1A. Front Mol Neurosci. 2017;10:299 pubmed publisher
Ruhe F, Olling A, Abromeit R, Rataj D, Grieschat M, Zeug A, et al. Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB. Front Cell Infect Microbiol. 2017;7:67 pubmed publisher
Alexopoulou Z, Lang J, Perrett R, Elschami M, Hurry M, Kim H, et al. Deubiquitinase Usp8 regulates ?-synuclein clearance and modifies its toxicity in Lewy body disease. Proc Natl Acad Sci U S A. 2016;113:E4688-97 pubmed publisher
Deng H, Shi Y, Yang Y, Ahmeti K, Miller N, Huang C, et al. Identification of TMEM230 mutations in familial Parkinson's disease. Nat Genet. 2016;48:733-9 pubmed publisher
Koschut D, Richert L, Pace G, Niemann H, Mely Y, Orian Rousseau V. Live cell imaging shows hepatocyte growth factor-induced Met dimerization. Biochim Biophys Acta. 2016;1863:1552-8 pubmed publisher
Yin X, Ambardekar C, Lu Y, Feng Z. Distinct Entry Mechanisms for Nonenveloped and Quasi-Enveloped Hepatitis E Viruses. J Virol. 2016;90:4232-4242 pubmed publisher
Gillespie L, Roosendahl P, Ng W, Brooks A, Reading P, Londrigan S. Endocytic function is critical for influenza A virus infection via DC-SIGN and L-SIGN. Sci Rep. 2016;6:19428 pubmed publisher
Schwartz V, Friedrich K, Polleichtner G, Gründer S. Acid-sensing ion channel (ASIC) 4 predominantly localizes to an early endosome-related organelle upon heterologous expression. Sci Rep. 2015;5:18242 pubmed publisher
Das U, Wang L, Ganguly A, Saikia J, Wagner S, Koo E, et al. Visualizing APP and BACE-1 approximation in neurons yields insight into the amyloidogenic pathway. Nat Neurosci. 2016;19:55-64 pubmed publisher
Yu C, Rafiq N, Cao F, Zhou Y, Krishnasamy A, Biswas K, et al. Integrin-beta3 clusters recruit clathrin-mediated endocytic machinery in the absence of traction force. Nat Commun. 2015;6:8672 pubmed publisher
Alekov A. Mutations associated with Dent's disease affect gating and voltage dependence of the human anion/proton exchanger ClC-5. Front Physiol. 2015;6:159 pubmed publisher
Cruse G, Beaven M, Music S, Bradding P, Gilfillan A, Metcalfe D. The CD20 homologue MS4A4 directs trafficking of KIT toward clathrin-independent endocytosis pathways and thus regulates receptor signaling and recycling. Mol Biol Cell. 2015;26:1711-27 pubmed publisher
Guo S, Liang Y, Murphy S, Huang A, Shen H, Kelly D, et al. A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications. Autophagy. 2015;11:560-72 pubmed publisher
Davidson A, Humphreys D, Brooks A, Hume P, Koronakis V. The Arf GTPase-activating protein family is exploited by Salmonella enterica serovar Typhimurium to invade nonphagocytic host cells. MBio. 2015;6: pubmed publisher
Sun J, Lu F, He H, Shen J, Messina J, Mathew R, et al. STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion. J Cell Biol. 2014;207:535-48 pubmed publisher
Yi P, Chew L, Zhang Z, Ren H, Wang F, Cong X, et al. KIF5B transports BNIP-2 to regulate p38 mitogen-activated protein kinase activation and myoblast differentiation. Mol Biol Cell. 2015;26:29-42 pubmed publisher
Loskutov Y, Kozyulina P, Kozyreva V, Ice R, Jones B, Roston T, et al. NEDD9/Arf6-dependent endocytic trafficking of matrix metalloproteinase 14: a novel mechanism for blocking mesenchymal cell invasion and metastasis of breast cancer. Oncogene. 2015;34:3662-75 pubmed publisher
Kimura T, Murakami F. Evidence that dendritic mitochondria negatively regulate dendritic branching in pyramidal neurons in the neocortex. J Neurosci. 2014;34:6938-51 pubmed publisher
Tsika E, Glauser L, Moser R, Fiser A, Daniel G, Sheerin U, et al. Parkinson's disease-linked mutations in VPS35 induce dopaminergic neurodegeneration. Hum Mol Genet. 2014;23:4621-38 pubmed publisher
Weir D, Laing E, Smith I, Wang L, Broder C. Host cell virus entry mediated by Australian bat lyssavirus G envelope glycoprotein occurs through a clathrin-mediated endocytic pathway that requires actin and Rab5. Virol J. 2014;11:40 pubmed publisher
Stafa K, Tsika E, Moser R, Musso A, Glauser L, Jones A, et al. Functional interaction of Parkinson's disease-associated LRRK2 with members of the dynamin GTPase superfamily. Hum Mol Genet. 2014;23:2055-77 pubmed publisher
He J, Sun E, Bujny M, Kim D, Davidson M, Zhuang X. Dual function of CD81 in influenza virus uncoating and budding. PLoS Pathog. 2013;9:e1003701 pubmed publisher
Roberts R, Al Jamal W, Whelband M, Thomas P, Jefferson M, Van den Bossche J, et al. Autophagy and formation of tubulovesicular autophagosomes provide a barrier against nonviral gene delivery. Autophagy. 2013;9:667-82 pubmed publisher
Jin R, Zhu W, Cao S, Chen R, Jin H, Liu Y, et al. Japanese encephalitis virus activates autophagy as a viral immune evasion strategy. PLoS ONE. 2013;8:e52909 pubmed publisher
Giger E, Castagner B, Räikkönen J, Mönkkönen J, Leroux J. siRNA transfection with calcium phosphate nanoparticles stabilized with PEGylated chelators. Adv Healthc Mater. 2013;2:134-44 pubmed publisher
Kalia M, Khasa R, Sharma M, Nain M, Vrati S. Japanese encephalitis virus infects neuronal cells through a clathrin-independent endocytic mechanism. J Virol. 2013;87:148-62 pubmed publisher
Gao Y, Bertuccio C, Balut C, Watkins S, Devor D. Dynamin- and Rab5-dependent endocytosis of a Ca2+ -activated K+ channel, KCa2.3. PLoS ONE. 2012;7:e44150 pubmed publisher
Smith K, Muir J, Rao Y, Browarski M, Gruenig M, Sheehan D, et al. Stabilization of GABA(A) receptors at endocytic zones is mediated by an AP2 binding motif within the GABA(A) receptor ?3 subunit. J Neurosci. 2012;32:2485-98 pubmed publisher
Atkin T, Brandon N, Kittler J. Disrupted in Schizophrenia 1 forms pathological aggresomes that disrupt its function in intracellular transport. Hum Mol Genet. 2012;21:2017-28 pubmed publisher
Ramonet D, Podhajska A, Stafa K, Sonnay S, Trancikova A, Tsika E, et al. PARK9-associated ATP13A2 localizes to intracellular acidic vesicles and regulates cation homeostasis and neuronal integrity. Hum Mol Genet. 2012;21:1725-43 pubmed publisher
Milev M, Brown C, Mouland A. Live cell visualization of the interactions between HIV-1 Gag and the cellular RNA-binding protein Staufen1. Retrovirology. 2010;7:41 pubmed publisher
Vonderheit A, Helenius A. Rab7 associates with early endosomes to mediate sorting and transport of Semliki forest virus to late endosomes. PLoS Biol. 2005;3:e233 pubmed
product information
Catalog Number :
14437
Product Name :
mRFP-Rab5
article :
doi10.1371/journal.pbio.0030233
id1267
pubmed_id15954801
bacterial resistance :
Kanamycin
cloning :
backbonepmRFP-C3
backbone_mutation
backbone_origin
backbone_size4656
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.
origin :
37
pi :
alt_names
Rab5
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5GACTACACCATCGTGGAA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRAB5
geneRAB5A
id5868
genbank_ids
NM_004162
mutation
nameRab 5
shRNA_sequence
size663
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmRFP
resistance markers :
198
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA