This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
TFORF2095
catalog :
141918
citations: 1
Reference
Joung J, Ma S, Tay T, Geiger Schuller K, Kirchgatterer P, Verdine V, et al. A transcription factor atlas of directed differentiation. Cell. 2023;186:209-229.e26 pubmed publisher
product information
Catalog Number :
141918
Product Name :
TFORF2095
article :
doi10.1016/j.cell.2022.11.026
id28211165
pubmed_id36608654
bacterial resistance :
Ampicillin
cloning :
backbonepLX_TRC317
backbone_mutation
backbone_originBroad Institute Genetic Perturbation Platform
backbone_size8264
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
NM_014682,XM_011517633,XM_017014063,XM_017014058,XM_011517632,XM_017014061,XM_017014071,XM_017014067,XM_017014054,XM_017014065,XM_017014053,XM_017014068,XM_017014048,XM_017014047,XM_017014051,XM_017014069,XM_017014064,XM_011517636,XM_017014062,XM_017014070,XM_011517635,XM_011517634,XM_017014066,XM_011517631,XM_011517629,XM_006716487,XM_011517637,XM_017014056,XM_017014052,XM_017014049,XM_017014057,XM_017014060,XM_017014059,XM_017014050,XM_017014055,XM_011517638,ENST00000276480 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at help@addgene.org prior to placing an order to request the full sequence.
growth strain :
Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3CACATAGCGTAAAAGGAGCAACATAG
sequencing_primer_5GGGTGGAGACTGAAGTTAGGCCAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNZF3, ZC2HC10, ZNF387
geneST18
id9705
genbank_ids
mutation
nameST18
shRNA_sequence
size3245
species
9606
Homo sapiens
tags
resistance markers :
898
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA