TFORF0033 | Addgene 141485 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

TFORF0033

catalog :

141485

citations: 1

Reference
Joung J, Ma S, Tay T, Geiger Schuller K, Kirchgatterer P, Verdine V, et al. A transcription factor atlas of directed differentiation. Cell. 2023;186:209-229.e26 pubmed publisher

product information

Catalog Number :

141485

Product Name :

TFORF0033

article :

doi	10.1016/j.cell.2022.11.026
id	28211165
pubmed_id	36608654

bacterial resistance :

Ampicillin

cloning :

backbone

pLX_TRC317

backbone_mutation

backbone_origin

Broad Institute Genetic Perturbation Platform

backbone_size

8264

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

NM_003109,XM_011538696,ENST00000426431 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at help@addgene.org prior to placing an order to request the full sequence.

growth strain :

Lentiviral vector for overexpressing the SP1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	EF1a
sequencing_primer_3	CACATAGCGTAAAAGGAGCAACATAG
sequencing_primer_5	GGGTGGAGACTGAAGTTAGGCCAG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	SP1
id	6667

genbank_ids

mutation

name

SP1

shRNA_sequence

size

2438

species

9606

Homo sapiens