This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FUW-tetO-SOX9
catalog :
141404
citations: 1
Reference
Tchieu J, Calder E, GUTTIKONDA S, Gutzwiller E, Aromolaran K, Steinbeck J, et al. NFIA is a gliogenic switch enabling rapid derivation of functional human astrocytes from pluripotent stem cells. Nat Biotechnol. 2019;37:267-275 pubmed publisher
product information
Catalog Number :
141404
Product Name :
FUW-tetO-SOX9
article :
doi10.1038/s41587-019-0035-0
id28208486
pubmed_id30804533
bacterial resistance :
Ampicillin
cloning :
backboneFUW-tetO
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
FUW-tetO-GFP (Addgene 30130) was digested with EcoRI to remove the GFP fragment and SOX9 was inserted
growth strain :
Inducible Expression of SOX9
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoR1
promoter
sequencing_primer_3FUW_R taaagcagcgtatcc
sequencing_primer_5FUW_F aactatgcgctcggg
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCMD1, CMPD1, SRA1, SRXX2, SRXY10
geneSOX9
id6662
genbank_ids
mutation
nameSOX9
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
3770
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA