This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro
catalog :
141390
citations: 7
Reference
Kim I, Lee Y, Khalid M, Chen I, Zhang Y, Ott M, et al. SARS-CoV-2 protein ORF8 limits expression levels of Spike antigen and facilitates immune evasion of infected host cells. J Biol Chem. 2023;299:104955 pubmed publisher
Lee M, Leong M, Rustagi A, Beck A, Zeng L, Holmes S, et al. SARS-CoV-2 escapes direct NK cell killing through Nsp1-mediated downregulation of ligands for NKG2D. Cell Rep. 2022;41:111892 pubmed publisher
Kee J, Thudium S, Renner D, Glastad K, Palozola K, Zhang Z, et al. SARS-CoV-2 disrupts host epigenetic regulation via histone mimicry. Nature. 2022;610:381-388 pubmed publisher
Hall R, Gued xe1 n A, Yap M, Young G, Harvey R, Stoye J, et al. SARS-CoV-2 ORF6 disrupts innate immune signalling by inhibiting cellular mRNA export. PLoS Pathog. 2022;18:e1010349 pubmed publisher
Beaudoin Bussieres G, Arduini A, Bourassa C, Medjahed H, Gendron Lepage G, Richard J, et al. SARS-CoV-2 Accessory Protein ORF8 Decreases Antibody-Dependent Cellular Cytotoxicity. Viruses. 2022;14: pubmed publisher
Rauti R, Shahoha M, Leichtmann Bardoogo Y, Nasser R, Paz E, Tamir R, et al. Effect of SARS-CoV-2 proteins on vascular permeability. elife. 2021;10: pubmed publisher
Gordon D, Jang G, Bouhaddou M, Xu J, Obernier K, White K, et al. A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. 2020;583:459-468 pubmed publisher
product information
Catalog Number :
141390
Product Name :
pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro
article :
doi10.1038/s41586-020-2286-9
id28211149
pubmed_id32353859
bacterial resistance :
Ampicillin
cloning :
backbonepLVX-EF1alpha-IRES-Puro
backbone_mutation
backbone_originTakara Bio
backbone_size8825
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2020.03.22.002386v3 for bioRxiv preprint.
growth strain :
Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
origin :
30
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterEF1alpha
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGU280_gp09
geneORF8
id43740577
genbank_ids
mutationhuman codon optimized
nameSARS-CoV-2-orf8
shRNA_sequence
size
species
32644
Other
SARS-CoV-2
tags
locationC terminal on insert
tag2xStrep
resistance markers :
4914
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA