pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro | Addgene 141387 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro

catalog :

141387

citations: 7

Reference
Krachmarova E, Petkov P, Lilkova E, Ilieva N, Rangelov M, Todorova N, et al. Insights into the SARS-CoV-2 ORF6 Mechanism of Action. Int J Mol Sci. 2023;24: pubmed publisher
Wang Z, Pan Q, Ma L, Zhao J, McIntosh F, Liu Z, et al. Anthracyclines inhibit SARS-CoV-2 infection. Virus Res. 2023;334:199164 pubmed publisher
Lee M, Leong M, Rustagi A, Beck A, Zeng L, Holmes S, et al. SARS-CoV-2 escapes direct NK cell killing through Nsp1-mediated downregulation of ligands for NKG2D. Cell Rep. 2022;41:111892 pubmed publisher
Hall R, Gued xe1 n A, Yap M, Young G, Harvey R, Stoye J, et al. SARS-CoV-2 ORF6 disrupts innate immune signalling by inhibiting cellular mRNA export. PLoS Pathog. 2022;18:e1010349 pubmed publisher
Yoo J, Sasaki M, Cho S, Kasuga Y, Zhu B, Ouda R, et al. SARS-CoV-2 inhibits induction of the MHC class I pathway by targeting the STAT1-IRF1-NLRC5 axis. Nat Commun. 2021;12:6602 pubmed publisher
Rauti R, Shahoha M, Leichtmann Bardoogo Y, Nasser R, Paz E, Tamir R, et al. Effect of SARS-CoV-2 proteins on vascular permeability. elife. 2021;10: pubmed publisher
Gordon D, Jang G, Bouhaddou M, Xu J, Obernier K, White K, et al. A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. 2020;583:459-468 pubmed publisher

product information

Catalog Number :

141387

Product Name :

pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro

article :

doi	10.1038/s41586-020-2286-9
id	28211149
pubmed_id	32353859

bacterial resistance :

Ampicillin

cloning :

backbone

pLVX-EF1alpha-IRES-Puro

backbone_mutation

backbone_origin

Takara Bio

backbone_size

8825

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Please visit https://www.biorxiv.org/content/10.1101/2020.03.22.002386v3 for bioRxiv preprint.

growth strain :

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	EF1alpha
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	GU280_gp06
gene	ORF6
id	43740572

genbank_ids

mutation

human codon optimized

name

SARS-CoV-2-orf6

shRNA_sequence

size

species

32644

Other

SARS-CoV-2