pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro | Addgene 141386 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

catalog :

141386

citations: 6

Reference
Lee M, Leong M, Rustagi A, Beck A, Zeng L, Holmes S, et al. SARS-CoV-2 escapes direct NK cell killing through Nsp1-mediated downregulation of ligands for NKG2D. Cell Rep. 2022;41:111892 pubmed publisher
Pringle E, Duguay B, Bui Marinos M, Mulloy R, Landreth S, Desireddy K, et al. Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virions. PLoS Pathog. 2022;18:e1010832 pubmed publisher
Lawanprasert A, Simonson A, Sumner S, Nicol M, Pimcharoen S, Kirimanjeswara G, et al. Inhalable SARS-CoV-2 Mimetic Particles Induce Pleiotropic Antigen Presentation. Biomacromolecules. 2022;23:1158-1168 pubmed publisher
Rauti R, Shahoha M, Leichtmann Bardoogo Y, Nasser R, Paz E, Tamir R, et al. Effect of SARS-CoV-2 proteins on vascular permeability. elife. 2021;10: pubmed publisher
Meng B, Kemp S, Papa G, Datir R, Ferreira I, Marelli S, et al. Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the Alpha variant B.1.1.7. Cell Rep. 2021;35:109292 pubmed publisher
Gordon D, Jang G, Bouhaddou M, Xu J, Obernier K, White K, et al. A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. 2020;583:459-468 pubmed publisher

product information

Catalog Number :

141386

Product Name :

pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

article :

doi	10.1038/s41586-020-2286-9
id	28211149
pubmed_id	32353859

bacterial resistance :

Ampicillin

cloning :

backbone

pLVX-EF1alpha-IRES-Puro

backbone_mutation

backbone_origin

Takara Bio

backbone_size

8825

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Please visit https://www.biorxiv.org/content/10.1101/2020.03.22.002386v3 for bioRxiv preprint.

growth strain :

Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	EF1alpha
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	GU280_gp05
gene	M
id	43740571

genbank_ids

mutation

human codon optimized

name

SARS-CoV-2-M

shRNA_sequence

size

species

32644

Other

SARS-CoV-2