This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLD-puro-Cc-TARDBP-WT_VA
catalog :
141327
citations: 1
Reference
Pourhaghighi R, Ash P, Phanse S, Goebels F, Hu L, Chen S, et al. BraInMap Elucidates the Macromolecular Connectivity Landscape of Mammalian Brain. Cell Syst. 2020;10:333-350.e14 pubmed publisher
product information
Catalog Number :
141327
Product Name :
pLD-puro-Cc-TARDBP-WT_VA
article :
doi10.1016/j.cels.2020.03.003
id28211137
pubmed_id32325033
bacterial resistance :
Ampicillin
cloning :
backbonepLD-puro-Cc-VA (MAPLE vector)
backbone_mutation
backbone_originAddgene# 24588
backbone_size9389
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-WT in mammalian cells
growth temp :
Grow for no longer than 16 hr
origin :
30
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_35'-TCTTTCCCCTGCACTGTACC-3'
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS10, TDP-43
geneTARDBP
id23435
genbank_ids
23435
mutation
nameTARDBP-WT
shRNA_sequence
size1608
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagVA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)
resistance markers :
3055
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA