pcDNA3 Flag TSC2 (S939A) | Addgene 14132 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3 Flag TSC2 (S939A)

catalog :

14132

citations: 2

Reference
Fidalgo da Silva E, Botsford S, Dare Shih J, Hanna M, Porter L. The Tuberin and Cyclin B1 complex functions as a novel G2/M sensor of serum conditions and Akt signaling. PLoS ONE. 2019;14:e0210612 pubmed publisher
Manning B, Tee A, Logsdon M, Blenis J, Cantley L. Identification of the tuberous sclerosis complex-2 tumor suppressor gene product tuberin as a target of the phosphoinositide 3-kinase/akt pathway. Mol Cell. 2002;10:151-62 pubmed

product information

Catalog Number :

14132

Product Name :

pcDNA3 Flag TSC2 (S939A)

article :

doi	10.1016/S1097-2765(02)00568-3
id	1260
pubmed_id	12150915

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3-Flag

backbone_mutation

backbone_origin

FLAG (MDYKDDDDK) was cloned between HindIII and BamHI of pcDNA3

backbone_size

5500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The first 276 base pairs of TSC2 were PCR-amplified from pcDNA6-TSC2 and inserted between the BamHI and NotI sites in frame with the FLAG-coding sequence in pcDNA3-FLAG. The remainder of TSC2 was subcloned from pcDNA6-TSC2 and inserted between the NotI and XbaI sites of the above construct. pcDNA3-FLAG-tuberinS939A was generated by site-directed PCR mutagenesis.

origin :

pi :

alt_names

tuberin

TSC2

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	BamHI
promoter
sequencing_primer_3	SP6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	LAM, PPP1R160, TSC4
gene	TSC2
id	7249

genbank_ids

mutation

S939A.

name

TSC2 S939A

shRNA_sequence

size

5400

species

9606

Homo sapiens